D-GLUCARIC ACID AND GALACTARIC ACID CATABOLISM BY AGROBACTERIUM-TUMEFACIENS
D-GLUCARIC ACID AND GALACTARIC ACID CATABOLISM BY AGROBACTERIUM-TUMEFACIENS
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DOI：
10.1128/jb.102.1.85-96.1970
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发表时间：
1970-01-01
影响因子：
3.2
通讯作者：
FEINGOLD, DS
中科院分区：
文献类型：
--
作者：
CHANG, YF;FEINGOLD, DS
农杆菌培养的无细胞提取物（粗提取物）-葡萄糖醛酸盐和-葡萄糖醛酸盐将-葡萄糖醛酸盐和半乳糖酸盐转化为2-酮-3-脱氧和4-脱氧-5-酮-d-葡萄糖醛酸盐的混合物。然后通过部分纯化的粗提取物将这些化合物转化为暂定鉴定为2,5-二酮己二酸酯的中间体。同样的酶制剂进一步使该中间体脱羧为α-酮戊二酸半醛，随后在烟酰胺腺嘌呤二核苷酸依赖反应中氧化为α-酮戊二酸。SinceA。tummefaciens将-葡萄糖醛酸转化为-葡萄糖酸，从-葡萄糖酸到α-酮戊二酸a的途径。tumefacienswas确定。
Cell-free extract (crude extract) ofAgrobacterium tumefaciensgrown ond-glucuronate ord-glucarate convertsd-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to α-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to α-ketoglutaric acid. SinceA. tumefaciensconvertsd-glucuronic acid tod-glucarate, a pathway fromd-glucuronate to α-ketoglutarate inA. tumefacienswas determined.