Cutaneous T-cell lymphoma: neoplasm of T cells with helper activity.
Cutaneous T-cell lymphoma: neoplasm of T cells with helper activity.
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皮肤 T 细胞淋巴瘤：具有辅助活性的 T 细胞肿瘤。
DOI：
10.1182/blood.v53.4.642.bloodjournal534642
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发表时间：
1979
期刊：
影响因子：
20.3
通讯作者：
R. L. Edelson
中科院分区：
文献类型：
--
作者：
Carole L. Berger;Dorothy Warburton;Jahangir Raafat;Paul LoGerfo;R. L. Edelson
对3例非白细胞性皮肤T细胞淋巴瘤患者的肿瘤性淋巴细胞进行了研究，以确定其肿瘤性T细胞是否具有辅助活性。也就是说，促进正常人B细胞合成免疫球蛋白的能力。从受累淋巴结中分离出肿瘤性T细胞的同质群体，核型分析显示三例中有两例为单克隆起源。通过E-玫瑰花结形成和随后的Ficoll-Hypaque浮选将来自对照外周血的单核细胞分离成T和B细胞富集部分。将商陆促分裂原刺激的正常和恶性T细胞与正常B细胞共培养。用荧光素标记的山羊抗人免疫球蛋白对细胞离心的细胞制备物进行染色，以定量产生免疫球蛋白的B细胞。向对照B细胞级分中加入对照T细胞级分使标记细胞的数量从8增加到34。肿瘤性T细胞增加了正常B细胞的细胞质免疫球蛋白的平均值为1 - 25，表现出特殊的辅助活性。这些结果支持Szary综合征之间的关系。先前证明是辅助性T细胞白血病和非白细胞性皮肤T细胞淋巴瘤，并表明恶性辅助性T细胞对皮肤具有独特的亲和力。
Neoplastic lymphocytes from 3 patients with aleukemic cutaneous T-cell lymphoma were studied to determine if their neoplastic T cells had helper activity. i.e., ability to promote immunoglobulin synthesis by normal human B cells. Homogeneous populations of neoplastic T cells were isolated from involved lymph nodes and in two of three instances were shown to be of monoclonal origin by karyotypic analysis. Mononuclear leukocytes from control peripheral blood were separated into Tand B-cellenriched fractions by E-rosette formation and subsequent Ficoll-Hypaque flotation. Pokeweed-mitogen-stimulated normal and malignant T cells were co-cultured with normal B cells. Cytocentrifuged cell preparations were stained with fluoresceinconjugated goat antihuman immunoglobulin to quantitate immunoglobulin-producing B cells. Addition of control T-cell fractions to control B-cell fractions increased the number of labeled cells from 8 to 34. Neoplastic T cells increased the numbers of normal B cells containing cytoplasmic immunoglobulin to a mean of 1 25, demonstrating exceptional helper activity. These results support a relationship between the S#{233}zarysyndrome. previously demonstrated to be a leukemia of helper T cells, and aleukemic cutaneous T-cell lymphoma and suggest that malignant helper T cells have a distinct affinity for the skin.