Maintenance of a constitutive heterochromatin domain in vertebrates by a Dicer-dependent mechanism.
Maintenance of a constitutive heterochromatin domain in vertebrates by a Dicer-dependent mechanism.
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DOI：
10.1038/ncb2010
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发表时间：
2010-01
影响因子：
21.3
通讯作者：
Felsenfeld, Gary
中科院分区：
文献类型：
--
作者：
Giles, Keith E.;Ghirlando, Rodolfo;Felsenfeld, Gary
本文利用鸡β-珠蛋白基因座与叶酸受体基因之间的16 kb异染色质结构域研究RNA依赖机制和组蛋白修饰在维持组成性异染色质结构中的作用。HDAC活性的抑制显示出既增加基因间转录又使异染色质更容易被MspI消化。我们表明，siRNA介导的酶切丁下调具有类似的效果：组蛋白乙酰化增加，转录水平上升，紧凑的染色质结构变得更容易限制性内切酶。我们还表明，鸡argonaute 2同源物结合的16 kb区域中的切丁机依赖的方式，是必要的浓缩染色质结构。这种异染色质结构域广泛分布于脊椎动物基因组中，因此似乎通过先前鉴定的高度保守的机制以其浓缩形式维持。
The 16 kb heterochromatin domain between the chicken β-globin locus and the folate receptor gene is used here to study the roles of RNA dependent mechanisms and histone modifications in maintenance of a constitutively heterochromatic structure. Inhibition of HDAC activity is shown to both increase intergenic transcription and render the heterochromatin more accessible to MspI digestion. We show that siRNA-mediated down regulation of the enzyme dicer has similar effects: histone acetylation is increased, transcript levels rise, and the compact chromatin structure becomes more accessible to restriction endonucleases. We also show that the chicken argonaute 2 homolog binds the 16 kb region in a dicer dependent manner and is necessary for a condensed chromatin structure. Heterochromatic domains of this kind, widely distributed in vertebrate genomes, thus appear to be maintained in their condensed form by previously identified and highly conserved mechanisms.
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DOI：
10.1073/pnas.94.2.575
发表时间：
1997-01-21
影响因子：
11.1
作者：
Chung, JH;Bell, AC;Felsenfeld, G
通讯作者：
Felsenfeld, G
影响因子：
30.8
作者：
Noma, K;Sugiyama, T;Grewal, SIS
通讯作者：
Grewal, SIS
影响因子：
56.9
作者：
Morris, KV;Chan, SWL;Looney, DJ
通讯作者：
Looney, DJ
影响因子：
11.4
作者：
Prioleau, MN;Nony, P;Felsenfeld, G
通讯作者：
Felsenfeld, G
影响因子：
4.5
作者：
Giles, KE;Caputi, M;Beemon, KL
通讯作者：
Beemon, KL