Pig skin structure and transdermal delivery of liposomes: A two photon microscopy study
Pig skin structure and transdermal delivery of liposomes: A two photon microscopy study
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DOI：
10.1016/j.jconrel.2008.08.006
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发表时间：
2008-11-24
影响因子：
10.8
通讯作者：
Bagatolli, Luis A.
中科院分区：
文献类型：
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作者：
Carrer, Dolores C.;Vermehren, Charlotte;Bagatolli, Luis A.
在这项工作中，我们表征了猪表皮的结构和物理特性，包括其对不同脂质体制剂的渗透性。自发荧光图像显示，表皮中的细胞从基底层到角质层组织成簇，而这些簇又被我们称为“峡谷”的特定结构分开。这些峡谷从表面的皱纹开始，最终闭合并一直进入表皮内部，成为到达基底层的独特结构。这种结构先前在小鼠皮肤的表皮中被描述为“簇间通路”，被认为充满了未知材料，并且对囊泡渗透的阻力较低。对猪皮肤的 LAURDAN 广义偏振图像的分析表明，峡谷中充满了非极性的低水合物质，类似于在猪皮肤角质层中观察到的情况。这些结果与从皮肤自发荧光图像获得的数据一起表明这些峡谷是 SC 物质的内陷/延伸。荧光标记的脂质融入非常灵活的脂质体中，能够渗透到皮肤中，最终到达基底层和真皮层。脂质体中带电脂质的存在增强了尺寸稳定性，从而增强了渗透效率。 (C) 2008 Elsevier B.V. 保留所有权利。
In this work we have characterized the architecture and physical properties of pig skin epidermis including its permeability to different liposome formulations. Autofluorescence images show that cells in the epidermis, from the basal layer to the stratum corneum, are organized in clusters that are in turn separated by particular structures we named "canyons". These canyons start in the surface as a wrinkle, eventually closing and going all the way inside the epidermis as a distinct structure that reaches the stratum basale. This structure, described previously in the epidermis of mouse skin as "intercluster pathway", was suggested to be filled with an unknown material and offer low resistance to vesicle penetration. Analysis of LAURDAN Generalized Polarization images of pig skin show that the canyons are filled with a non-polar poorly hydrated material, similar to that observed in pig skin stratum corneum. These results together with the data obtained from skin autofluorescence images suggest that these canyons are invaginations/extension of SC material. Fluorescently labeled lipids incorporated into very flexible liposomes are able to penetrate into the skin, eventually reaching the basal layer and the dermis plane. The presence of charged lipids in the liposomes enhances size stability and thus the efficiency of penetration. (C) 2008 Elsevier B.V. All rights reserved.