Infrared attenuated total reflection (IR-ATR) spectroscopy for detecting drugs in human saliva
Infrared attenuated total reflection (IR-ATR) spectroscopy for detecting drugs in human saliva
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DOI：
10.1002/dta.346
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发表时间：
2012-06-01
影响因子：
2.9
通讯作者：
Sigrist, Markus W.
中科院分区：
文献类型：
--
作者：
Hans, Kerstin M. -C.;Mueller, Susanne;Sigrist, Markus W.
毒品的消费在世界范围内引起了极大的关注。已经开发了各种用于人体的药物测试，但还没有紧凑和易于使用的测试设备可用于现场直接半定量药物测试。我们建议使用衰减全反射(ATR)红外光谱作为一种传感方法来分析人类唾液样本中的药物。在这篇文章中，我们给出了在2300到900 cm-1的红外范围内的ATR光谱，作为迈向这种器件的第一步。我们重点研究了常见药物可卡因及其代谢物，并研究了选定的稀释剂、掩蔽剂、常见药物和软饮料的光谱干扰问题。此外，还记录了唾液样本的光谱，并给出了饮酒后光谱特征随时间的变化。据我们所知，这是第一次不仅讨论了目标药物(可卡因)在水和唾液中溶解的光谱，而且还讨论了受试者唾液样本中可能存在的干扰化合物的光谱。本文提出了吸收可卡因(包括其代谢物)最合适的光谱范围，并将被研究物质的干扰降至最低。该光谱窗口位于1800-1710 cm-1之间。此外，我们还证明了用红外光谱检测唾液中0.020 mg/ml的可卡因是可行的，而不需要任何分离或提取过程。例如，这项技术也可以应用于废水中的药物检测。版权所有(C)2011 John Wiley&Sons，Ltd.
The consumption of drugs is of great concern worldwide. Various drug tests for humans have been developed but there is no compact and easy-to-use test device available yet for direct semi-quantitative drug testing in the field. We suggest using attenuated total reflection (ATR) infrared spectroscopy as a sensing method to analyze human saliva samples with respect to drugs. In this paper, we present ATR spectra in the infrared range between 2300 and 900?cm-1 as a first step towards such a device. We emphasize the common drug cocaine and its metabolites and investigate the problems of spectral interferences of selected diluents, masking agents, common medication, and soft drinks. Furthermore, spectra of saliva samples are recorded and a time-dependent change of the spectral signatures after alcohol consumption is presented. To the best of our knowledge, it is the first time that not only spectra of the drug of interest (cocaine) dissolved in water and in saliva but also spectra of interfering compounds possibly present in the saliva sample of a tested subject are discussed. This paper presents the most appropriate spectral range for strong cocaine absorption (including its metabolites) and minimum interference by the investigated substances. This spectral window is found to be between 1800 and 1710?cm-1. In addition, we demonstrate the feasibility to identify cocaine in saliva at a concentration of 0.020?mg/ml with IR-ATR-spectroscopy without any separation or extraction procedures. For example, this technique could also be applied for drug detection in waste water. Copyright (c) 2011 John Wiley & Sons, Ltd.