IN-VIVO PRODUCTION OF INTERLEUKIN-10 BY NON-T CELLS IN RHEUMATOID-ARTHRITIS, SJOGRENS-SYNDROME, AND SYSTEMIC LUPUS-ERYTHEMATOSUS - A POTENTIAL MECHANISM OF B-LYMPHOCYTE HYPERACTIVITY AND AUTOIMMUNITY
IN-VIVO PRODUCTION OF INTERLEUKIN-10 BY NON-T CELLS IN RHEUMATOID-ARTHRITIS, SJOGRENS-SYNDROME, AND SYSTEMIC LUPUS-ERYTHEMATOSUS - A POTENTIAL MECHANISM OF B-LYMPHOCYTE HYPERACTIVITY AND AUTOIMMUNITY
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DOI：
10.1002/art.1780371114
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发表时间：
1994-11-01
影响因子：
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通讯作者：
EMILIE, D
中科院分区：
文献类型：
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作者：
LLORENTE, L;RICHAUDPATIN, Y;EMILIE, D
Objective.白细胞介素-10（IL-10）是一种有效的体外B淋巴细胞刺激因子。因此，在类风湿关节炎（RA），原发性干燥综合征（SS）和系统性红斑狼疮（SLE）患者体内IL-10基因表达失调进行了分析。采用逆转录聚合酶链反应和酶联免疫吸附试验检测10例未经治疗的RA、SS和SLE患者及15例正常对照者外周血单个核细胞自发产生IL-10的情况。RA、SS和SLE患者IL-10的产生显著高于对照组。各组中B淋巴细胞和单核细胞均产生IL-10，而T淋巴细胞不产生。IL-10的产生在RA、SS和SLE中增加。它可能在B淋巴细胞过度活跃和自身免疫的发展中起作用。
Objective. Interleukin-10 (IL-10) is a potent stimulator of B lymphocytes in vitro. In vivo dysregulation of IL-10 gene expression was therefore analyzed in patients with rheumatoid arthritis (RA), primary Sjogren's syndrome (SS), and systemic lupus erythematosus (SLE).Methods. Spontaneous production of IL-10 by peripheral blood mononuclear cells was measured using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay in untreated patients with either RA (n = 10), SS (n = 10), or SLE (n = 10), and in 15 normal control subjects.Results. IL-10 production was dramatically higher in RA, SS, and SLE patients than in controls. In each group, both B lymphocytes and monocytes, but not T lymphocytes, produced IL-10.Conclusion. IL-10 production is increased in RA, SS, and SLE. It may play a role in B lymphocyte hyperactivity and in the development of autoimmunity.