Diet-induced maternal obesity alters ovarian morphology and gene expression in the adult mouse offspring
Diet-induced maternal obesity alters ovarian morphology and gene expression in the adult mouse offspring
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DOI：
10.1016/j.fertnstert.2014.06.015
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发表时间：
2014-09-01
影响因子：
6.7
通讯作者：
Cagampang, Felino R.
中科院分区：
文献类型：
--
作者：
Cheong, Ying;Sadek, Khaled H.;Cagampang, Felino R.
目的：研究高脂（HF）饮食诱导的母体肥胖对成年后代卵巢中卵泡群和基因表达的影响。设计：实验小鼠研究。设置：实验室。动物：HF饮食的小鼠。干预：雌性C57 BL/6 J小鼠在受孕前6周、整个妊娠期和哺乳期喂食HF或标准食物（C）饮食。后代从断奶开始喂食C或HF饮食，创建HF/HF，HF/C，C/HF，C/C后代groups.Main Outcome Measures（s）：卵泡计数和成年后代卵巢中的基因表达。结果（s）：产前暴露于母体HF营养导致后代卵巢中原始，窦和Graafian卵泡数量减少（HF/C和HF/HF）。凋亡相关基因的表达水平（FoXO 3 α）、卵泡生长和发育（Gdf 9）以及昼夜节律产生（Clock和Bmal 1）在HF/C和HF/HF后代的卵巢中升高，而昼夜节律钟基因Cry 1和Per 1在HF/HF后代的卵巢中表达较低。母亲怀孕期间的肥胖对成年后代卵巢中的卵泡生长和发育具有长期的有害后果，这可能会影响其生殖潜力。(C)2014年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To examine the effects of high-fat (HF) diet-induced maternal obesity on follicular population and gene expression in adult offspring ovaries.Design: Experimental mouse study.Setting: Laboratory.Animal(s): Mice on HF diet.Intervention(s): Female C57BL/6J mice were fed an HF or standard chow (C) diet 6 weeks before conception, through pregnancy and lactation. Offspring were fed the C or HF diet from weaning, creating the HF/HF, HF/C, C/HF, C/C offspring groups.Main Outcome Measure(s): Follicular counts and gene expression in adult offspring ovaries.Result(s): Prenatal exposure to maternal HF nutrition resulted in the reduction of primordial, antral, and Graafian follicle numbers in offspring ovaries (both HF/C and HF/HF). Expression levels of genes involved in apoptosis (FoXO3 alpha), follicular growth and development (Gdf9), and circadian rhythms generation (Clock and Bmal1) were elevated in the ovaries of HF/C and HF/HF offspring, while expression of the circadian clock genes Cry1 and Per1 were lower in HF/HF ovaries.Conclusion(s): Maternal obesity during pregnancy has long-term deleterious consequences on follicular growth and development in the adult offspring ovaries, which may impact their reproductive potential. (C) 2014 by American Society for Reproductive Medicine.