Regulation of lipoprotein lipase in muscle and adipose tissue during exercise.
Regulation of lipoprotein lipase in muscle and adipose tissue during exercise.
复制标题
运动过程中肌肉和脂肪组织中脂蛋白脂肪酶的调节。
DOI：
10.1152/jappl.1991.71.2.404
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Palmer,WK
中科院分区：
文献类型：
--
作者：
Ladu,MJ;Kapsas,H;Palmer,WK
脂蛋白脂酶(LPL)是以组织特异性的方式调节的；运动增加肌肉中的LPL活性，同时降低脂肪组织中的LPL活性。本研究旨在探讨一次运动后大鼠肌肉和脂肪组织中LPL活性与LPL mRNA表达的关系。游泳2小时后，与安静对照组动物相比，运动组动物脂肪组织中的LPL活性降低了43%(110+/-26到63+/-17)，比目鱼肌中的LPL活性几乎增加了一倍(203+/-26到383+/-59)。同时，脂肪组织中LPL基因的表达降低了42%，红股和白股肌肉中LPL基因的表达分别增加了50%和100%。游泳24小时后，脂肪组织和比目鱼肌中的LPL活性已恢复到控制水平。运动后24小时，运动组大鼠脂肪组织中LPL mRNA的表达仍下降23%，但运动组和对照组的肌肉组织中LPL mRNA的表达差异无统计学意义。总RNA浓度的变化不能解释LPL基因相对表达的变化。运动后0、24 h肌肉和脂肪组织LPL酶活性与LPL m RNA的相关系数分别为+0.86和+0.93。因此，运动引起的酶活性的组织特异性变化可能部分通过翻译前控制来调节。
Lipoprotein lipase (LPL) is regulated in a tissue-specific manner; exercise increases LPL activity in muscle at the same time it is reduced in adipose tissue. The purpose of this study was to determine the relationship between LPL activity and LPL mRNA in muscle and adipose tissue in rats exposed to one bout of exercise. Immediately after a 2-h swim, LPL activity [pmol free fatty acids (FFA).min-1.mg tissue-1] in the exercised animals was reduced 43% in adipose tissue (110 +/- 26 to 63 +/- 17) and increased almost twofold in the soleus muscle (203 +/- 26 to 383 +/- 59) compared with sedentary control animals. At the same time, LPL mRNA was reduced 42% in adipose tissue and increased 50 and 100% in the red vastus and white vastus muscles, respectively. Twenty-four hours after the swim, LPL activity had returned to control levels in adipose tissue and the soleus muscle. At hour 24 of recovery, LPL mRNA was still reduced 23% in the adipose tissue of exercised animals but was not significantly different between exercised and control animals in any of the muscle tissues analyzed. Changes in total RNA concentration could not account for the changes in relative LPL mRNA expression. The relationship between LPL enzyme activity and LPL mRNA in muscle and adipose tissue was +0.86 and +0.93 at 0 and 24 h postexercise, respectively. Thus the tissue-specific changes in enzyme activity induced by exercise could be mediated, in part, through pretranslational control.