The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo
The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo
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DOI：
10.1152/ajpgi.2001.280.5.g922
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发表时间：
2001-05-01
影响因子：
4.5
通讯作者：
Holm, L
中科院分区：
文献类型：
--
作者：
Atuma, C;Strugala, V;Holm, L
关于胃肠道黏液层厚度和外观的体外研究结果存在分歧，此前已有报道。通过体内模型，我们研究了从胃到结肠黏液凝胶厚度随时间的变化。麻醉后的大鼠胃肠道组织经腔面朝上放置，进行活体显微镜观察。用微移液管测量吸痰前后黏液的厚度。黏液层呈半透明连续状；结肠最厚（与830妈妈相似），空肠最薄（与123妈妈相似）。去除黏液后，连续的、牢固粘附的黏液层仍然附着在体（类似于80 mum）、上颌窦（类似于154 mum）和结肠（类似于116 mum）的上皮表面。在小肠中，这一层很薄（类似于20mm）或不存在。去除黏液后，黏液厚度持续增加，结肠黏液厚度增加率最高，胃黏液厚度增加率最低。综上所述，胃肠道黏液凝胶在体内的黏附是连续的，可分为两层，一层是可被吸出的松散黏附层，另一层是牢固附着在粘膜上的层。
Divergent results from in vitro studies on the thickness and appearance of the gastrointestinal mucus layer have previously been reported. With an in vivo model, we studied mucus gel thickness over time from stomach to colon. The gastrointestinal tissues of Inactin-anesthetized rats were mounted luminal side up for intravital microscopy. Mucus thickness was measured with a micropipette before and after mucus removal by suction. The mucus layer was translucent and continuous; it was thickest in the colon (similar to 830 mum) and thinnest in the jejunum (similar to 123 mum). On mucus removal, a continuous, firmly adherent mucus layer remained attached to the epithelial surface in the corpus (similar to 80 mum), antrum (similar to 154 mum), and colon (similar to 116 mum). In the small intestine, this layer was very thin (similar to 20 mum) or absent. After mucus removal, there was a continuous increase in mucus thickness with the highest rate in the colon and the lowest rate in the stomach. In conclusion, the adherent gastrointestinal mucus gel in vivo is continuous and can be divided into two layers: a loosely adherent layer removable by suction and a layer firmly attached to the mucosa.