IFT-81 and IFT-74 are required for intraflagellar transport in C-elegans
IFT-81 and IFT-74 are required for intraflagellar transport in C-elegans
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DOI：
10.1111/j.1365-2443.2007.01076.x
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发表时间：
2007-05-01
期刊：
影响因子：
2.1
通讯作者：
Mitani, Shohei
中科院分区：
文献类型：
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作者：
Kobayashi, Tetsuo;Gengyo-Ando, Keiko;Mitani, Shohei
在许多真核和原核细胞中，鞭毛内运输（IFT）是纤毛生物发生和维持的重要机制。已知有大量多肽参与IFT，但每种成分的生理作用尚未完全阐明。在这里，我们鉴定了秀丽隐杆线虫衣藻IFT成分的同源物IFT-81，并发现其功能丧失突变体表现出不寻常的行为特性和较小的体型。IFT-81在感觉神经元中表达，定位于纤毛基部。在一些IFT突变体中也观察到与IFT -81突变体相似的表型，表明这些缺陷是由IFT无法引起的。我们还证明了IFT-81与IFT-74相互作用并共定位，IFT-74是IFT的另一个假定成分。功能缺失突变体ift-74表现出与ift-81突变体相同的表型，表明ift-81和ift-74具有相似的功能。此外，ift-81和ift-74突变体在纤毛形成方面同样表现出微弱的异常，在成熟纤毛中也表现出明显的运输中断。因此，我们认为IFT-81和IFT-74在线虫感觉纤毛的IFT中协同作用。
Intraflagellar transport (IFT) is essential machinery for biogenesis and maintenance of cilia in many eukaryotic and prokaryotic cells. A large number of polypeptides are known to be involved in IFT, but the physiological role of each component is not fully elucidated. Here, we identified a C. elegans orthologue of a Chlamydomonas reinhardtii IFT component, IFT-81, and found that its loss-of-function mutants show an unusual behavioral property and small body size. IFT-81 is expressed in sensory neurons, and localized at the base of cilia. The similar phenotypes with ift-81 mutants were also observed in several IFT mutants, suggesting these defects are caused by inability of IFT. We also demonstrated that IFT-81 interacts and co-localizes with IFT-74, which is another putative component of IFT. The ift-74 loss-of-function mutants showed phenocopies with ift-81 mutants, suggesting IFT-81 and IFT-74 play comparable functions. Moreover, ift-81 and ift-74 mutants similarly exhibited weak anomalies in cilia formation and obvious disruptions of transport in mature cilia. Thus, we conclude that IFT-81 and IFT-74 coordinately act in IFT in C. elegans sensory cilia.