A preliminary investigation of metal element profiles in the serum of patients with bloodstream infections using inductively-coupled plasma mass spectrometry (ICP-MS)
A preliminary investigation of metal element profiles in the serum of patients with bloodstream infections using inductively-coupled plasma mass spectrometry (ICP-MS)
复制标题
使用电感耦合等离子体质谱 (ICP-MS) 对血流感染患者血清中金属元素谱进行初步研究
DOI：
10.1016/j.cca.2018.07.013
复制
发表时间：
2018
影响因子：
5
通讯作者：
Lei Li
中科院分区：
文献类型：
--
作者：
Suying Zhao;Shuyuan Cao;Lan Luo;Zhan Zhang;Gehui Yuan;Yanan Zhang;Yanting Yang;Weihui Guo;Li Wang;Feng Chen;Qian Wu;Lei Li
背景我们通过电感耦合等离子体质谱（ICP-MS）测定了血流感染（BSI）患者血清中的金属元素谱（MEP），并找出了特定感染中非常重要（VIP）的金属元素。方法从14名葡萄球菌感染BSI患者的血清和分离的细菌中鉴定出68种金属元素，39 ICP-MS 方法检测肠杆菌科感染者、肠球菌感染者 5 例、无感染者 58 例。采用统计分析、主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）对不同组别数据进行处理，选择差异金属元素并进行相关性分析。结果BSI患者血清中4种细菌（金黄色葡萄球菌、大肠杆菌、屎肠球菌、克雷伯菌）的MEPs 肺炎），并建立了细菌相应的 MEP。在不同BSI患者中筛选出VIP金属元素。相关分析显示血清金属元素浓度与细菌感染之间存在一定相关性。结论我们发现BSI患者血清中金属元素与对照组相比存在差异,从而从金属组学角度为BSI的诊断、预防和治疗提供依据。
BackgroundWe determined metal element profiles (MEPs) by inductively-coupled plasma mass spectrometry (ICP-MS) in the serum of patients with blood stream infection (BSI) and find out very important (VIP) metal elements in specific infections.MethodsSixty-eight metal elements were identified in both serum and the bacteria isolated from 14 BSI patients withStaphylococcusinfection, 39 withEnterobacteriaceaeinfection, 5 withEnterococcusinfection and 58 healthy subjects without infection by ICP-MS methods. Statistical analysis, Principal Component Analysis (PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) were performed to process data among different groups, select differential metal elements and operate correlation analysis.ResultsThe MEPs in the serum of BSI patients with 4 types of bacteria (Staphylococcus aureus,Escherichia coli,Enterococcus faecium,andKlebsiella pneumonia), and the corresponding MEPs of the bacteria were established. VIP metal elements were screened out in different BSI patients. Correlation analysis showed that there were some correlations between serum concentrations of metal elements and bacterial infection.ConclusionWe found differential metal elements in the serum of BSI patients compared with controls, thus providing a basis for the diagnosis, prevention and treatment of BSI from the perspective of metallomics.