Neural correlates of beneficial effects of young plasma treatment in aged mice: PET-SPM analyses and neuro-behavioural/molecular biological studies
Neural correlates of beneficial effects of young plasma treatment in aged mice: PET-SPM analyses and neuro-behavioural/molecular biological studies
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年轻血浆治疗对老年小鼠有益效果的神经相关性：PET-SPM 分析和神经行为/分子生物学研究
DOI：
10.1007/s00259-021-05598-4
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发表时间：
2021
影响因子：
9.1
通讯作者：
Watanabe Y
中科院分区：
文献类型：
--
作者：
Zhang K;Mizuma H;Nakatani Y;Kanayama Y;Takahashi K;Matsumoto Y;Wada Y;Onoe K;Owada S;Hayashinaka E;Wu Y;Zhang X;Tian M;Zhang H;Watanabe Y
目的研究青年血浆（young blood plasma，YBP）对老年小鼠神经功能的影响，并探讨YBP在体内外治疗老年小鼠神经功能损伤的分子机制。治疗4周后进行清醒状态下的体内2-[18 F]-氟-2-脱氧-d-葡萄糖正电子发射断层扫描（18 F-FDG PET）和认知行为测试。此外，在体外衰老模型的建立，和关键的认知相关蛋白的表达和/或关键神经元通路的改变进行了分析，在脑组织和培养cells.ResultsAged小鼠用YBP治疗表现出较高的葡萄糖代谢在右侧海马和双侧躯体感觉皮质，和较低的葡萄糖代谢在右侧床核的终纹和左侧小脑。YBP治疗对空间和长期社会识别记忆产生了有益的影响，并显着增加了几种认知相关蛋白的表达，并改变了海马和体感皮层中的关键神经元信号通路。进一步的体外研究表明，YBP，而不是老年血浆显着上调几个认知相关的proteins.ConclusionOur结果突出的作用，海马和躯体感觉皮层在YBP诱导的有益效果，对识别记忆在老年mice.18F-FDG PET成像下的意识状态，为探索YBP治疗对年龄相关的认知能力下降的机制提供了一个新的途径。
PurposeTo investigate the in vivo neurofunctional changes and therapeutic effects of young blood plasma (YBP) in aged mice, as well as the molecular mechanisms underlying the therapeutic effects of YBP ex vivo and in vitro.MethodsAged C57/BL6 mice received systemic administrations of phosphate-buffered saline (PBS) or YBP twice a week, for 4 weeks. In vivo 2-[18F]-fluoro-2-deoxy-d-glucose positron emission tomography (18F-FDG PET) under conscious state and cognitive behavioural tests were performed after 4-week treatment. In addition, an in vitro senescent model was established, and the expressions of key cognition-associated proteins and/or the alterations of key neuronal pathways were analysed in both brain tissues and cultured cells.ResultsAged mice treated with YBP demonstrated higher glucose metabolism in the right hippocampus and bilateral somatosensory cortices, and lower glucose metabolism in the right bed nucleus of stria terminalis and left cerebellum. YBP treatment exerted beneficial effects on the spatial and long-term social recognition memory, and significantly increased the expressions of several cognition-related proteins and altered the key neuronal signalling pathways in the hippocampus and somatosensory cortex. Further in vitro studies suggested that YBP but not aged blood plasma significantly upregulated the expressions of several cognition-associated proteins.ConclusionOur results highlight the role of the hippocampus and somatosensory cortex in YBP-induced beneficial effects on recognition memory in aged mice.18F-FDG PET imaging under conscious state provides a new avenue for exploring the mechanisms underlying YBP treatment against age-related cognitive decline.