Cleavage analysis of actinophage R4 and its deletion mutants.
Cleavage analysis of actinophage R4 and its deletion mutants.
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噬菌体 R4 及其缺失突变体的裂解分析。
DOI：
10.1080/00021369.1983.10865878
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
H. Saito
中科院分区：
文献类型：
--
作者：
T. Morino;T. Isogai;H. Kojima;Hideo Takahashi;H. Saito
我们构建了 PstI、PvuII、EcoRI、SeaI、KpnI、BglII 和 BclI 的放线菌 R4 限制性酶切图谱。 R4 噬菌体的 DNA 没有 7 种限制性内切酶 BamHI、ClaI、HindIII、SacII、SlaI (XhoI) 或 XbaI 的切割位点。 R4 噬菌体的整个基因组大小估计为 53.7 KB。 R4 噬菌体的三个缺失被划定在 R4 基因组上。所有三个缺失均位于 R4 限制性图谱的右侧。
We have constructed restriction maps of actinophage R4 for PstI, PvuII, EcoRI, SeaI, KpnI, BglII and BclI. DNA of R4 phage had no cleavage sites for seven restriction endonucleases, BamHI, ClaI, HindIII, SacII, SlaI (XhoI) or XbaI. The entire genome size of the R4 phage was estimated to be 53.7 kilobases. Three deletions of the R4 phage were delimitated on the R4 genome. All three deletions were located on the right hand side of the R4 restriction map.