Transcriptional patterns of Emiliania huxleyi in the North Pacific Subtropical Gyre reveal the daily rhythms of its metabolic potential
Transcriptional patterns of Emiliania huxleyi in the North Pacific Subtropical Gyre reveal the daily rhythms of its metabolic potential
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DOI：
10.1111/1462-2920.14855
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发表时间：
2019-11-27
影响因子：
5.1
通讯作者：
Dyhrman, Sonya T.
中科院分区：
文献类型：
--
作者：
Limon, Maria D. Hernandez;Hennon, Gwenn M. M.;Dyhrman, Sonya T.
Emiliania huxleyi是一种钙化附着植物，对海洋有机和无机碳循环都有贡献。在海洋生态系统中，光是浮游植物生理学的主要驱动力，并最终通过生态系统的碳流。在这里，我们分析了在北太平洋副热带环流（NPSG）中收集的元转录组的拉格朗日时间序列，以研究E。huxleyi通过昼夜转换调节基因表达。许多E. Huxleyi重叠群具有昼夜表达模式，61%的重叠群聚集成具有统计学显著昼夜周期性的模块。参与能量储存过程的重叠群，如碳固定和脂质合成，在黎明前后达到高峰。相反，参与释放能量储存过程的重叠群，如呼吸和脂质降解，在中午和黄昏达到峰值。这些模式表明了一个精心策划的循环，即在E.在NPSG的huxleyi种群。选定的重叠群相关的细胞周期也表现出显着的昼夜周期性与培养中观察到的分裂相调节一致。总的来说，这些基因表达模式表明，在这个营养耗尽的生态系统中，每天的代谢级联可能有助于有机和无机碳的流动。
Emiliania huxleyi is a calcifying haptophyte, contributing to both the organic and inorganic marine carbon cycles. In marine ecosystems, light is a major driver of phytoplankton physiology and ultimately carbon flow through the ecosystem. Here, we analysed a Lagrangian time-series of metatranscriptomes collected in the North Pacific Subtropical Gyre (NPSG) to examine how in situ populations of E. huxleyi modulate gene expression over day-night transitions. Many E. huxleyi contigs had a diel expression pattern, with 61% of contigs clustering into modules with statistically significant diel periodicity. Contigs involved in processes that build up energy stores, like carbon fixation and lipid synthesis, peaked around dawn. In contrast, contigs involved in processes that released energy stores, like respiration and lipid degradation, peaked mid-day and towards dusk. These patterns suggest an orchestrated cycle of building, then consuming energy stores in E. huxleyi populations in the NPSG. Selected contigs related to the cell cycle also exhibited significant diel periodicity consistent with phased modulations of division observed in culture. Overall, these patterns of gene expression suggest a daily metabolic cascade that could contribute to both organic and inorganic carbon flow in this nutrient depleted ecosystem.