In vivo assessment of behavioral recovery and circulatory exchange in the peritoneal parabiosis model.
In vivo assessment of behavioral recovery and circulatory exchange in the peritoneal parabiosis model.
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DOI：
10.1038/srep29015
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发表时间：
2016-07-01
影响因子：
4.6
通讯作者：
Wyss-Coray T
中科院分区：
文献类型：
--
作者：
Castellano JM;Palner M;Li SB;Freeman GM Jr;Nguyen A;Shen B;Stan T;Mosher KI;Chin FT;de Lecea L;Luo J;Wyss-Coray T
通过一种被称为联体共生的外科手术，两只动物之间共享循环，为我们理解生理学创造了大量信息，最近在神经科学竞技场。全身环境是组织、免疫细胞和循环分子的复杂储存库，令人惊讶的是，就其跨器官系统的通信而言，还没有很好地理解。虽然该模型已被用于探测复杂的生理问题多年，恢复和交换动力学的关键参数仍然不完全的特点，限制了设计实验和解释复杂问题的结果的能力。在这里，我们提供的证据表明，小鼠加入联体逐渐恢复许多生理相关的脑功能的研究。具体来说，我们描述了各种恢复参数的时间过程，包括一般健康和代谢，运动协调，活动和睡眠行为。最后，我们描述了几个淋巴细胞群体的嵌合体的动力学，以及小分子的摄取到小鼠的大脑后联体。我们的表征为那些试图了解免疫系统与大脑以及其他器官系统之间复杂相互作用的人提供了重要资源。
The sharing of circulation between two animals using a surgical procedure known as parabiosis has created a wealth of information towards our understanding of physiology, most recently in the neuroscience arena. The systemic milieu is a complex reservoir of tissues, immune cells, and circulating molecules that is surprisingly not well understood in terms of its communication across organ systems. While the model has been used to probe complex physiological questions for many years, critical parameters of recovery and exchange kinetics remain incompletely characterized, limiting the ability to design experiments and interpret results for complex questions. Here we provide evidence that mice joined by parabiosis gradually recover much physiology relevant to the study of brain function. Specifically, we describe the timecourse for a variety of recovery parameters, including those for general health and metabolism, motor coordination, activity, and sleep behavior. Finally, we describe the kinetics of chimerism for several lymphocyte populations as well as the uptake of small molecules into the brains of mice following parabiosis. Our characterization provides an important resource to those attempting to understand the complex interplay between the immune system and the brain as well as other organ systems.