Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2
Schwann cells induce Proliferation and Migration of Oligodendrocyte Precursor Cells Through Secretion of PDGF-AA and FGF-2
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雪旺细胞通过分泌 PDGF-AA 和 FGF-2 诱导少突胶质细胞前体细胞的增殖和迁移
DOI：
10.1007/s12031-015-0570-1
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发表时间：
2015-08-01
影响因子：
3.1
通讯作者：
Hu, Jian-Guo
中科院分区：
文献类型：
--
作者：
Chen, Yue-Juan;Zhang, Jing-Xing;Hu, Jian-Guo
我们之前的研究表明，共移植雪旺细胞（SC）促进移植少突胶质细胞前体细胞（OPC）的增殖和迁移。然而，共接枝 SC 如何影响 OPC 尚未明确。在本研究中，我们使用 SC 条件培养基 (SCM) 证实 SC 诱导的 OPC 增殖和迁移是由 SC 分泌因子介导的。然后，我们在 SC 和 SCM 中检测到了几个候选因子：PDGF-AA、FGF-2 和 IGF-1，以及它们在 OPC 中的受体。最后，通过使用选择性抑制剂，检查了这些候选因子对OPCs增殖和迁移的影响。我们的结果表明，AG1295（PDGFR 抑制剂）和 PD173074（FGFR 抑制剂）均可显着降低 SCM 刺激的 OPC 增殖和迁移。总之，我们的研究表明 SC 通过分泌 PDGF-AA 和 FGF-2 影响 OPC 的增殖和迁移。这些分子的识别不仅有助于了解 SC 诱导的 OPC 增殖和迁移的机制，而且为中枢神经系统疾病的治疗提供可能的靶点。
Our previous study has showed that co-grafted Schwann cells (SCs) promote proliferation and migration of the grafted oligodendrocyte precursor cells (OPCs). However, how the co-grafted SCs affect OPCs has not been clarified. In the present study, we confirmed that SC-induced proliferation and migration of OPCs were mediated by SC-secreted factors using SC-conditioned medium (SCM). Then, we detected several candidate factors, PDGF-AA, FGF-2, and IGF-1, in SCs and SCM, and their receptors in OPCs. Finally, by using the selective inhibitors, the effects of these candidate factors on proliferation and migration of OPCs were examined. Our results showed that SCM-stimulated proliferation and migration of OPCs could be markedly decreased by both AG1295 (the inhibitor of PDGFR) and PD173074 (the inhibitor of FGFR). Together, our study suggests that SCs affect proliferation and migration of OPCs through secreting PDGF-AA and FGF-2. Identity of these molecules not only contributes to understand the mechanism of SC-induced proliferation and migration of OPCs but also provides possible target for treatment of CNS diseases.