Diversification of cardiomyogenic cell lineages in vitro.
Diversification of cardiomyogenic cell lineages in vitro.
复制标题
体外心肌细胞谱系的多样化。
DOI：
10.1006/dbio.1995.1234
复制
发表时间：
1995
影响因子：
2.7
通讯作者：
Bader,D
中科院分区：
文献类型：
--
作者：
Yutzey,K;Gannon,M;Bader,D
未分化的心源性中胚层的能力，以产生多样化的生肌表型进行了测定，在一个最小的培养系统。在体内心脏发生过程中，鸟类心脏的前段和后段在首次分化时具有不同的收缩蛋白基因表达模式。为了评估未分化的心源性组织在心脏形成之前分化成不同的前谱系和后谱系的潜力，在Hamburger和汉密尔顿阶段4-8将心源性中胚层和内胚层一起从胚胎中去除。从这些阶段中的每一个外植体分化在限定培养基中所示的肌肉特异性基因的表达。然而，表达心房特异性肌球蛋白重链（AMHC 1）mRNA的能力仅限于后心脏祖细胞。多样化不依赖于前内胚层，这表明中胚层和内胚层之间的诱导相互作用是没有必要的，以保持多样化的心脏谱系后，阶段4。维甲酸处理改变了再生心源性组织的多样性潜能，导致前祖细胞AMHC 1基因表达的激活。前心源性细胞在第8阶段从胚胎中取出，当心脏开始在体内分化时，不容易通过维甲酸处理改变多样化的表型。因此，在原肠胚形成后不久，前侧板中胚层中存在形成不同的心肌细胞谱系的潜力，并且这些谱系以位置依赖性方式的成熟在简单定义的体外培养系统中维持。
The ability of undifferentiated cardiogenic mesoderm to generate diversified myogenic phenotypes was assayed in a minimal culture system. During cardiogenesis in vivo, the anterior and posterior segments of the avian heart have distinct patterns of contractile protein gene expression when they first differentiate. To assess the potential of undifferentiated cardiogenic tissue to diversify into distinct anterior and posterior lineages prior to heart formation, cardiogenic mesoderm and endoderm were removed together from the embryo at Hamburger and Hamilton stages 4-8. Explants from each of these stages differentiated in defined medium as indicated by the expression of muscle-specific genes. However, the ability to express the atrial-specific myosin heavy chain (AMHC1) mRNA was confined to posterior cardiac progenitors. Diversification was not dependent on anterior endoderm, suggesting that inductive interactions between the mesoderm and endoderm are not necessary to maintain diversified cardiac lineages after stage 4. The diversified potential of explanted cardiogenic tissue was altered with retinoic acid treatment, resulting in the activation of AMHC1 gene expression in the anterior progenitors. Anterior cardiogenic cells removed from the embryo at stage 8, when the heart begins to differentiate in vivo, are not susceptible to the alteration of diversified phenotype by retinoic acid treatment. Therefore, the potential to form distinct cardiomyogenic cell lineages is present in the anterior lateral plate mesoderm soon after gastrulation and the maturation of these lineages in a positionally dependent manner is maintained in a simple defined culture system in vitro.