Structural Characterization of a Tetrapeptide from Sesame Flavor-Type Baijiu and Its Preventive Effects against AAPH-Induced Oxidative Stress in HepG2 Cells
Structural Characterization of a Tetrapeptide from Sesame Flavor-Type Baijiu and Its Preventive Effects against AAPH-Induced Oxidative Stress in HepG2 Cells
复制标题
芝麻香型白酒四肽的结构表征及其对AAPH诱导的HepG2细胞氧化应激的预防作用
DOI：
10.1021/acs.jafc.7b04815
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发表时间：
2017-12-06
影响因子：
6.1
通讯作者：
Sun, Baoguo
中科院分区：
文献类型：
--
作者：
Wu, Jihong;Huo, Jiaying;Sun, Baoguo
中国白酒中的肽很少被报道，因为它们通常以非常低的浓度存在于复杂的基质中。最近，通过高效液相色谱和四极杆飞行时间质谱（HPLC-QTOF-MS）从芝麻香型白酒中鉴定出一种四肽，Ala-Lys-Arg-Ala（AKRA），其浓度为8.497+/-0.753μg/L（P>0.05），该四肽具有预防作用。 对 2,2'-偶氮二(2-甲基丙脒)二盐酸盐 (AAPH) 诱导的 HepG2 细胞氧化应激的影响。细胞抗氧化活性测定结果表明，AKRA 以浓度依赖性方式保护 HepG2 细胞中 AAPH 诱导的氧化应激。用 AKRA (0.38-1.50 mg/mL) 预处理细胞 2 小时，引起强烈的细胞内活性氧 (ROS) 清除活性，并防止还原型谷胱甘肽 (GSH) 的减少以及氧化型谷胱甘肽 (GSSG) 和丙二醛 (MDA) 的增加。此外，AKRA 治疗还可防止 AAPH 诱导的谷胱甘肽过氧化物酶 (GSH-Px)、超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 显着下降。因此，AKRA 治疗改善了 HepG2 细胞中 AAPH 诱导的氧化应激。这项研究对于功能性白酒生产的设计和调控具有重要意义。
Peptides are rarely reported from Chinese Baijiu (Chinese liquor) because they are often present in very low concentrations in the complex matrix. A tetrapeptide, Ala-Lys-Arg-Ala (AKRA), was recently identified by high-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry (HPLC-QTOF-MS) from sesame flavor-type Baijiu at a concentration of 8.497 +/- 0.753 mu g/L (P > 0.05), and this tetrapeptide showed preventive effects against 2,2'-azobis(2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The cellular antioxidant activity assay results showed that AKRA protected AAPH-induced oxidative stress in HepG2 cells in a concentration-dependent manner. Pretreatment of the cells for 2 h with AKRA (0.38-1.50 mg/mL) caused strong intracellular reactive oxygen species (ROS) scavenging activities and prevented a decrease in reduced glutathione (GSH) and increases in oxidized glutathione (GSSG) and malondialdehyde (MDA). In addition, AKRA treatment prevented significant decreases in glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) induced by AAPH. Thus, AKRA treatment ameliorated AAPH-induced oxidative stress in HepG2 cells. This study will be important for the design and regulation of functional Baijiu production.