Assay methods for H2S biogenesis and catabolism enzymes.
Assay methods for H2S biogenesis and catabolism enzymes.
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DOI：
10.1016/bs.mie.2014.11.016
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发表时间：
2015
影响因子：
--
通讯作者：
Yadav PK
中科院分区：
文献类型：
--
作者：
Banerjee R;Chiku T;Kabil O;Libiad M;Motl N;Yadav PK
硫化氢是由含硫氨基酸、半胱氨酸和同型半胱氨酸或分解产物3-巯基丙酮酸通过三种已知的酶产生的：胱硫醚β-合成酶、γ-胱硫酮酶和3-巯基丙酮酸硫转移酶。其中，前两种酶存在于细胞质中，构成了跨硫途径，而第三种酶同时存在于细胞质和线粒体中。下列线粒体酶氧化硫化氢：硫代苯醌氧化还原酶、硫双加氧酶、罗丹宁和亚硫酸盐氧化酶。硫化物氧化途径的产物是硫代硫酸盐和硫酸盐。本章描述了参与硫化物产生和氧化清除硫代硫酸盐的酶的测定。
H2S is produced from sulfur-containing amino acids, cysteine and homocysteine, or a catabolite, 3-mercaptopyruvate, by three known enzymes: cystathionine β-synthase, γ-cystathionase, and 3-mercaptopyruvate sulfurtransferase. Of these, the first two enzymes reside in the cytoplasm and comprise the transsulfuration pathway, while the third enzyme is found both in the cytoplasm and in the mitochondrion. The following mitochondrial enzymes oxidize H2S: sulfide quinone oxidoreductase, sulfur dioxygenase, rhodanese, and sulfite oxidase. The products of the sulfide oxidation pathway are thiosulfate and sulfate. Assays for enzymes involved in the production and oxidative clearance of sulfide to thiosulfate are described in this chapter.