Culture of autopsy-derived fibroblasts as a tool to study systemic alterations in human neurodegenerative disorders such as Alzheimer's disease - methodological investigations
Culture of autopsy-derived fibroblasts as a tool to study systemic alterations in human neurodegenerative disorders such as Alzheimer's disease - methodological investigations
复制标题
DOI：
10.1007/s007020050177
复制
发表时间：
1999-01-01
影响因子：
3.3
通讯作者：
Ohm, TG
中科院分区：
文献类型：
--
作者：
Meske, V;Albert, F;Ohm, TG
本研究旨在探讨死后培养人成纤维细胞的可行性，将死后成纤维细胞与尸检证实的脑组织和组织病理学分期的脑组织相结合，可以进行动态生化的相关研究。系统性潜在或伴随神经和/或精神障碍的过程。这些研究局限于尸检脑或不确定当神经病理学状态缺乏时，即当成纤维细胞从活体患者获得时。我们对人尸检成纤维细胞和实验控制的大鼠死后条件下的成纤维细胞的检查清楚地表明，尸检来源的成纤维细胞可以可靠地培养。生长的细胞显示出典型的形态学和染色特征以及药理学。响应能力。甚至从99岁的个体或死后延迟48小时的个体获得的细胞也在我们的培养系统中生长。
The present study was undertaken in order to analyse the possibility of culturing post mortem derived human fibroblasts, The combination of post mortem fibroblasts with the autopsy proven and histopathologically staged brain will allow the correlative investigation of dynamic biochemical. processes which are systemically underlying or accompanying a neurological and/or psychiatric disorder. These studies are limited in autopsy brain or are uncertain when the neuropathological status is lacking, i.e. when fibroblasts were obtained from living patients. Our examinations of human autopsy fibroblast and those under experimentally controlled post mortem conditions with rats clearly demonstrate that autopsy-derived fibroblasts can be reliably cultured. The cells grown displayed typical morphological and staining characteristics as well as pharmacological. responsiveness. Even cells obtained from a 99 years old individual or an individual with a post mortem delay of 48 hours grew in our culture system.