Aryl Azide Photochemistry in Defined Protein Environments
Aryl Azide Photochemistry in Defined Protein Environments
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DOI：
10.1021/ol3028779
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发表时间：
2013-02-15
期刊：
影响因子：
5.2
通讯作者：
Tippmann, Eric M.
中科院分区：
文献类型：
--
作者：
Morris, Josephine L.;Reddington, Samuel C.;Tippmann, Eric M.
将基因编码的芳基氮烯前体对叠氮苯丙氨酸特定位点引入蛋白质中，以推断不同的环境是否能够捕获反应性有机中间体。突变型 T4 溶菌酶或绿色荧光蛋白光解后，EPR 光谱分别显示三重态氮宾和苯胺基的存在。
A genetically encoded precursor to an aryl nitrene, para-azidophenyialanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.