Ultrasonic activation of irrigants increases growth factor release from human dentine
Ultrasonic activation of irrigants increases growth factor release from human dentine
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DOI：
10.1007/s00784-016-1824-1
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发表时间：
2017-04-01
影响因子：
3.4
通讯作者：
Galler, K. M.
中科院分区：
文献类型：
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作者：
Widbiller, M.;Eidt, A.;Galler, K. M.
生物活性蛋白质被隔离在人类牙本质中，并在牙髓再生和修复中起决定性作用。它们可以通过酸释放并暴露在牙本质表面上，但也可以通过螯合剂如乙二胺四乙酸（EDTA）释放并暴露在牙本质表面上。本研究的目的是（i）评价超声激活根管内冲洗剂是否会促进牙本质中生长因子的释放;（ii）收集生理溶液中的生物活性蛋白。该方案包括用10%EDTA和磷酸盐缓冲盐水（PBS）通过单个或两个连续步骤处理，其中每个样品处理三次。为了模拟临床条件，选定的冲洗方案被应用到根管提取的人牙准备后。使用酶联免疫吸附试验定量溶液中转化生长因子β 1（TGF-β 1）的量。进行非参数统计分析以比较不同组以及组内的重复（Mann-Whitney U检验，α = 0.05）。此外，牙本质表面的形态学变化，通过扫描电子显微镜（SEM）。TGF-β 1检测不到后冲洗牙本质与PBS，无论是有或没有超声激活。EDTA冲洗释放TGF-β 1，超声激活EDTA增强了这种作用。然而，之前的EDTA调节使得生物活性蛋白能够释放到PBS溶液中。在牙本质盘和根管中观察到类似的结果。不同处理后的牙本质表面可视化显示，无论冲洗液超声激活后的浅表糜烂，但不同程度的有机物质的暴露。超声激活促进生长因子从人牙本质的释放。生物活性蛋白质可以在生理溶剂中分离，并可作为再生牙髓治疗或牙髓组织工程的自体补充物。
Bioactive proteins are sequestered in human dentine and play a decisive role in dental pulp regeneration and repair. They can be released and exposed on the dentine surface by acids, but also chelators, such as ethylenediaminetetraacetic acid (EDTA). The objectives of this study were (i) to evaluate whether ultrasonic activation of irrigants in the root canal will promote growth factor release from dentine and (ii) to collect bioactive proteins in a physiological solution.Human dentine disks underwent irrigation with and without ultrasonic activation. The protocols included treatment by either a single or two consecutive steps with 10 % EDTA and phosphate-buffered saline (PBS), where each sample was treated three times. To mimic clinical conditions, selected irrigation regimens were applied to root canals of extracted human teeth after preparation. Amounts of transforming growth factor beta 1 (TGF-beta 1) in solution were quantified using enzyme-linked immunosorbent assays. Nonparametric statistical analysis was performed to compare different groups as well as repetitions within a group (Mann-Whitney U test, alpha = 0.05). Additionally, morphological changes of dentine surfaces were visualized by scanning electron microscopy (SEM).TGF-beta 1 was not detectable after irrigation of dentine with PBS, neither with nor without ultrasonic activation. Irrigation with EDTA released TGF-beta 1, and ultrasonic activation of EDTA enhanced this effect. However, preceding EDTA conditioning enabled the release of bioactive proteins into PBS solution. Similar results were observed in dentine disks and root canals. Visualization of dentine surfaces after different treatment revealed superficial erosion after ultrasonic activation irrespective of the irrigant solution, but different degrees of exposure of organic substance.Ultrasonic activation enhances growth factor release from human dentine. Bioactive proteins can be isolated in physiological solvents and may act as autologous supplements for regenerative endodontic treatment or pulp tissue engineering.Autologous growth factors from human dentine can advance treatment strategies in dental pulp tissue engineering.