Extracellular Vesicles from Human Adipose-Derived Stem Cells for the Improvement of Angiogenesis and Fat-Grafting Application
Extracellular Vesicles from Human Adipose-Derived Stem Cells for the Improvement of Angiogenesis and Fat-Grafting Application
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人脂肪干细胞胞外囊泡促进血管生成及脂肪移植的研究
DOI：
10.1097/prs.0000000000006046
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发表时间：
2019-10-01
影响因子：
3.6
通讯作者：
Wang, Zhenxing
中科院分区：
文献类型：
--
作者：
Mou, Shan;Zhou, Muran;Wang, Zhenxing
背景资料：自体脂肪移植的效果会因脂肪吸收和纤维化而降低，这些与血管化不理想密切相关。细胞外囊泡是细胞分泌组的重要组成部分，能够反映其亲本细胞的功能和分子特征。越来越多的证据表明脂肪间充质干细胞具有促进血管形成的能力，其部分原因是细胞外囊泡的存在。作者评价了脂肪间充质干细胞衍生的细胞外囊泡是否改善了脂肪移植物的血管化并增加了其留存率。研究方法：为了检测脂肪间充质干细胞来源的细胞外囊泡的血管生成能力，从培养的人脂肪间充质干细胞的上清液中分离它们，并与人脐静脉内皮细胞体外孵育。然后，将囊泡与脂肪共移植到裸鼠皮下。移植后3个月，组织学观察移植物的留存率和炎症反应。结果：实验组在20 μ g/ml浓度下能显著促进细胞迁移和管形成。移植后3个月，实验组的体积（0.12 ± 0.03 mm（3））大于空白组（0.05 ± 0.01 mm（3））。组织学和免疫组织学结果显示，细胞外囊泡组的囊肿和空泡明显减少，纤维化程度减轻，新生血管增多。结论：作者将脂肪来源的间充质干细胞来源的细胞外囊泡与脂肪共同移植到裸鼠模型中，发现囊泡通过增强血管形成和调节炎症反应来改善体积保留。
Background: The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis that are closely related to unsatisfactory vascularization. Extracellular vesicles are key components of the cell secretome, which can mirror the functional and molecular characteristics of their parental cells. Growing evidence has revealed that adipose-derived mesenchymal stem cells have the ability to enhance vascularization, which is partly ascribed to extracellular vesicles. The authors evaluated whether adipose-derived mesenchymal stem cell-derived extracellular vesicles improved vascularization of fat grafts and increased their retention rate. Methods: To test the angiogenesis ability of adipose-derived mesenchymal stem cell-derived extracellular vesicles, they were isolated from the supernatant of cultured human adipose-derived mesenchymal stem cells and incubated with human umbilical vein endothelial cells in vitro. Then, the vesicles were co-transplanted with fat into nude mice subcutaneously. Three months after transplantation, the retention rate and inflammatory reaction of the grafts were analyzed by histologic assay. Results: The experimental group could significantly promote migration and tube formation at the concentration of 20 mu g/ml. At 3 months after transplantation, the volume of the experimental group (0.12 +/- 0.03 mm(3)) was larger compared with the blank group (0.05 +/- 0.01 mm(3)). Histology and immunohistology results demonstrated significantly fewer cysts and vacuoles, less fibrosis, and more neovessels in the extracelluar vesicle group. Conclusions: The authors co-transplanted adipose-derived mesenchymal stem cell-derived extracellular vesicles with fat into a nude mouse model and found that the vesicles improved volume retention by enhancing vascularization and regulating the inflammatory response.