PRENATAL INHIBITION OF AROMATASE-ACTIVITY AFFECTS LUTEINIZING-HORMONE FEEDBACK MECHANISMS AND REPRODUCTIVE BEHAVIORS OF ADULT GUINEA-PIGS
PRENATAL INHIBITION OF AROMATASE-ACTIVITY AFFECTS LUTEINIZING-HORMONE FEEDBACK MECHANISMS AND REPRODUCTIVE BEHAVIORS OF ADULT GUINEA-PIGS
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DOI：
10.1095/biolreprod51.6.1273
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发表时间：
1994-12-01
影响因子：
3.6
通讯作者：
RESKO, JA
中科院分区：
文献类型：
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作者：
CHOATE, JVA;RESKO, JA
通过用芳香化酶抑制剂1，4，6-雄甾三烯-3，17-二酮（ATD）从妊娠第30天至第55天处理妊娠豚鼠，研究脑芳香化对性别分化的必要性。在出生后第16周，受试者接受性腺切除术，并在用10 μ g苯甲酸雌二醇（EB）治疗后采集血样，用于引起LH峰;受试者随后接受GnRH治疗，以检测垂体反应性。用放射免疫法测定血浆LH。产前ATD治疗不影响男性或女性受试者LH峰机制的组织。Au动物，无论性别或治疗，释放LH后GnRH治疗，但促性腺激素对GnRH的反应减弱在子宫内ATD治疗的男性和女性。此外，在对GnRH刺激的反应中，LH释放的模式存在显著的性别差异，ATD处理的动物对EB对LW分泌的负反馈作用没有反应（p < 0.05），并且该组中显示前凸行为的雄性动物的百分比高于对照组（p < 0.05）。给药对雌性动物的坐骑行为和脊柱前凸行为无显著影响。这些数据表明，雌激素需要在组织的大脑区域，介导负反馈控制LH在两种性别和脊柱前凸行为的雄性豚鼠。控制LH的正反馈机制的组织似乎受到雄激素的控制。我们的数据还表明，促性腺激素细胞对GnRH作用的反应是由产前雌激素协调的，并且是性分化的。
The necessity of brain aromatization for sexual differentiation was investigated by treating pregnant guinea pigs with an aromatase inhibitor, 1,4,6-androstatriene-3,17-dione (ATD), from Day 30 to Day 55 of gestation. In postnatal Week 16, subjects were gonadectomized, and blood samples were collected after treatment with 10 mu g estradiol benzoate (EB), used to elicit an LH surge; subjects were subsequently treated with GnRH to test pituitary responsiveness. Plasma samples were assayed for LH by RIA. Prenatal ATD treatment did not affect the organization of the LH surge mechanism in either male or female subjects. AU animals, regardless of sex or treatment, released LH after GnRH treatment, but the responsiveness of the gonadotroph to GnRH was attenuated in both males and females treated with ATD in utero. In addition, a significant sex difference in the pattern of LH released in response to a GnRH challenge was found. ATD-treated animals did not respond to the negative feedback actions of EB on LW secretion (p < 0.05), and the percentage of males displaying lordosis behavior was greater in this group than in controls (p < 0.05). Mounting behavior and lordosis behavior of females were not significantly affected by treatment. These data demonstrate a need for estrogen in the organization of brain areas that mediate negative feedback control of LH in both sexes and lordosis behavior in the male guinea pig. The organization of positive feedback mechanisms for controlling LH seems to be under androgenic control. Our data also suggest that the responsiveness of the gonadotroph to GnRH action is developmentally coordinated by prenatal estrogen and is sexually differentiated.