Mechanism of atropine-resistant contraction induced by Dai-kenchu-to in guinea pig ileum
Mechanism of atropine-resistant contraction induced by Dai-kenchu-to in guinea pig ileum
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DOI：
10.1254/jjp.86.32
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发表时间：
2001-05-01
期刊：
影响因子：
--
通讯作者：
Nakamura, T
中科院分区：
文献类型：
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作者：
Satoh, K;Hashimoto, K;Nakamura, T
为阐明黛根藤的收缩作用机制，本实验研究了花椒有效成分羟基β-三七醇对豚鼠回肠无粘膜纵肌的收缩作用，并与花椒籽进行了比较。10(-7)-10(-5)g/ml的羟基β-山梨醇可引起剂量相关的自主收缩，在10(-4)g/ml或更高的浓度下可产生初始收缩。河豚毒素或辣椒素受体拮抗剂卡萨西平均能显著抑制羟基β-山梨醇(10(-5)g/ml)引起的收缩。尽管阿托品或P物质拮抗剂Spantide倾向于抑制收缩，但阿托品和Spantide合用可几乎完全阻断羟基β-山梨醇的收缩作用，而P-2-嘌呤受体拮抗剂pyridoxal-phosphate-6-azophenyl-2‘，4’-disulphonic酸不影响有或无Spantide的羟基β-山梨醇引起的收缩。花椒(2×10~(-4)g/m l)和黛根素(10(~(-3)g/m l)可显著抑制或倾向于被阿托品、Spantide、河豚毒素或卡萨西平抑制，并可被阿托品和Spantide合用显著抑制。提示胆碱能神经的乙酰胆碱释放和感觉神经元的速激肽参与了羟基β-三羟色胺的收缩反应，而速激肽可能参与了黛根藤的阿托品拮抗收缩作用。
To clarify the contractile mechanism of Dai-kenchu-to, the effects of hydroxy beta -sanshool tan ingredient of Zanthoxylum fruit), Zanthoxylum fruit (a constituent herb of Dai-kenchu-to) and Dai-kenchu-to were studied in mucosa-free longitudinal muscle of guinea pig ileum. Hydroxy beta -sanshool at 10(-7) - 10(-5) g/ml induced dose-related contractions accompanied by autonomous contraction and produced an initial contraction at a concentration of 10(-4) g/ml or more. The contraction induced by hydroxy beta -sanshool (10(-5) g/ml) was significantly inhibited by tetrodotoxin or the capsaicin-receptor antagonist capsazepine. Although atropine or the substance P antagonist spantide tended to inhibit the contraction, a combination of atropine and spantide almost abolished the contraction by hydroxy beta -sanshool, The P-2-purinoceptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid did not affect hydroxy beta -sanshool-induced contraction in the presence or absence of spantide. The tonic contractions by Zanthoxylum fruit (2 x 10(-4) g/ml) and Dai-kenchu-to (10(-3) g/ml) were significantly inhibited or tended to be inhibited by atropine, spantide, tetrodotoxin or capsazepine and were remarkably suppressed by the combination of atropine and spantide. These results suggested that acetylcholine release from intrinsic cholinergic nerves and tachykinins from sensory neurons are involved in the contractions induced by hydroxy beta -sanshool and that tachykinins may be involved in the atropine-resistant contraction by Dai-kenchu-to.