NC37-R1 Epstein-Barr virus (EBV): a possible recombinant between intracellular NC37 viral DNA and superinfecting P3HR-1 EBV.
NC37-R1 Epstein-Barr virus (EBV): a possible recombinant between intracellular NC37 viral DNA and superinfecting P3HR-1 EBV.
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NC37-R1 Epstein-Barr 病毒 (EBV)：细胞内 NC37 病毒 DNA 和重复感染的 P3HR-1 EBV 之间可能的重组体。
作者：
K. Fresen;M. Cho;L. Gissmann;H. zur Hausen
NC37-R1 细胞系是用从 P3HR-1 重复感染的 NC37 细胞中回收的 Epstein-Barr 病毒 (EBV) 转化人脐带血淋巴细胞后建立的，可自发产生具有转化但不具有早期抗原诱导特性的病毒颗粒。 NC37-R1的子代病毒目前已保留了病毒自发释放和转化的生物学特性，长达四个转化周期。用限制性内切酶 Hind III 切割后，对纯化的 NC37-R1 病毒粒子 DNA 进行分析，并使用印迹杂交技术将其片段与 P3HR-1 EBV 以及细胞内 NC37 病毒 DNA 进行比较，表明 NC37-R1 源自重复感染的 P3HR-1 和内源 NC37 EBV DNA 之间的重组。
The NC37-R1 cell line, established after transformation of human cord blood lymphocytes with Epstein-Barr virus (EBV) recovered from P3HR-1 superinfected NC37 cells, spontaneously produces viral particles with transforming but without early antigen-inducing properties. Progeny virus of NC37-R1 has retained its biological characteristics of spontaneous virus release and transformation up to four cycles of transformation at present. Analysis of purified NC37-R1 virion DNA, after cleavage with restriction endonuclease Hind III and comparison of its fragments with P3HR-1 EBV as well as intracellular NC37 viral DNA using the blot hybridization technique, suggests that NC37-R1 originates from a recombination between superinfecting P3HR-1 and endogenous NC37 EBV DNA.