A comparative physical study of two different hydrophilic synthetic latex matrices for the construction of a glucose biosensor
A comparative physical study of two different hydrophilic synthetic latex matrices for the construction of a glucose biosensor
复制标题
DOI：
10.1016/s0039-9140(01)00498-2
复制
发表时间：
2001-12-13
期刊：
影响因子：
6.1
通讯作者：
Rico-Lattes, I
中科院分区：
文献类型：
--
作者：
Cosnier, S;Szunerits, S;Rico-Lattes, I
由羟基(1)或葡萄糖酰胺(2)基团功能化的两种不同的可生物降解乳胶聚合物被证明是葡萄糖氧化酶的良好固定基质。为了在氧气存在下氧化葡萄糖酶促氧化产生的过氧化氢，这些生物传感器对葡萄糖添加的响应通过在0.6 V/SCE下修饰电极电位来测量。这种电极的响应被评价为薄膜厚度、pH和温度的函数。旋转圆盘电极实验表明，酶对两种乳胶膜的结构都有影响，即基质渗透率明显提高。基于乳胶1的酶传感器(双层，P-m = 8.10 x 10（-4) cm s(-1)）的高渗透性导致了高动态范围。此外，根据酶的构象，确定了乳胶1传感器的活化能分别为44.55和18.03 kJ mol（-1）。(C) 2001 Elsevier Science B.V.版权所有
Two different biodegradable latex polymers functionalised by hydroxy (1) or gluconamide (2) groups proved to be good immobilisation matrixes for glucose oxidase. The responses of these biosensors to glucose additions were measured by potentiostating the modified electrodes at 0.6 V/SCE in order to oxidise the hydrogen peroxide generated by the enzymatic oxidation of glucose in the presence of oxygen. The response of such electrodes was evaluated as a function of film thickness, pH and temperature. Rotating disk electrode experiments showed the influence of the enzyme on the structure of both latex films, namely a marked improvement in matrix permeability. The high permeability of the latex 1 based enzyme sensor (bilayer, P-m = 8.10 x 10(-4) cm s(-1)) resulted in a high dynamic range. Furthermore, the activation energy for a latex 1 sensor was determined to be 44.55 and 18.03 kJ mol(-1), respectively depending on the conformation of the enzyme. (C) 2001 Elsevier Science B.V. All rights reserved.