Directed assembly of discrete gold nanoparticle groupings using branched DNA scaffolds
Directed assembly of discrete gold nanoparticle groupings using branched DNA scaffolds
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DOI：
10.1021/cm0484089
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发表时间：
2005-04-05
影响因子：
8.6
通讯作者：
Alivisatos, AP
中科院分区：
文献类型：
--
作者：
Claridge, SA;Goh, SL;Alivisatos, AP
探索了自组装树枝状聚合物的概念，用于创建离散的纳米颗粒组装体。分支DNA三聚体和纳米颗粒-DNA缀合物的杂交导致纳米颗粒三聚体和四聚体复合物的合成。多个四聚体架构进行了研究，利用不同的二级结构基序的Au-DNA共轭物。通过凝胶电泳分析杂交产物，并且观察到对应于具有增加数目的杂交事件的结构的离散条带。从每个波段提取的样品进行了分析，通过透射电子显微镜，和统计数据编译的显微照片被用来比较每个架构的组装特性。也产生不对称结构，其中5-和10-nm Au颗粒组装在分支支架上。
The concept of self-assembled dendrimers is explored for the creation of discrete nanoparticle assemblies. Hybridization of branched DNA trimers and nanoparticle-DNA conjugates results in the synthesis of nanoparticle trimer and tetramer complexes. Multiple tetramer architectures are investigated, utilizing Au-DNA conjugates with varying secondary structural motifs. Hybridization products are analyzed by gel electrophoresis, and discrete bands are observed corresponding to structures with increasing numbers of hybridization events. Samples extracted from each band are analyzed by transmission electron microscopy, and statistics compiled from micrographs are used to compare assembly characteristics for each architecture. Asymmetric structures are also produced in which both 5- and 10-nm Au particles are assembled on branched scaffolds.