Quantitative analysis of clonidine and ephedrine by a microfluidic system: On-chip electromembrane extraction followed by high performance liquid chromatography
Quantitative analysis of clonidine and ephedrine by a microfluidic system: On-chip electromembrane extraction followed by high performance liquid chromatography
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DOI：
10.1016/j.jchromb.2017.10.062
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发表时间：
2017-11-15
影响因子：
3
通讯作者：
Karami, Monireh
中科院分区：
文献类型：
--
作者：
Baharfar, Mahroo;Yamini, Yadollah;Karami, Monireh
在这项工作中，微流控装置的芯片上电膜萃取微量麻黄碱（EPH）和可乐定（CLO）在人的尿液和血浆样品，然后进行HPLC-UV分析。使用两个聚甲基丙烯酸甲酯板作为基底，并且在每个板中雕刻微通道。下板上的微通道通道提供样品溶液的流动通道，上板上的微通道专用于停滞受体相的隔室。用有机溶剂浸渍一片聚丙烯片，并将其安装在芯片器件的两个部分之间。通过放置在通道底部的两个嵌入式铂电极产生穿过多孔片的电场，所述电极连接到电源。分析物被转化为它们的离子化形式，通过支撑液膜，然后通过施加的电压提取到受体相。所有的有效参数，包括SLM的类型，SLM的组成，给体和受体相的pH值，和施加的电压的量进行了评估和优化。评价几种有机溶剂作为SLM以评估SLM组合物的效果。其他参数通过中心复合设计进行优化。在最佳条件下，电压为74 V，流速为28 μ L·min（-1），100和20 mM HCl分别作为受体相和供体相的组成，绘制了两种分析物的校准曲线。检测限分别小于7.0和11 μ g L-1的尿液和血浆中，分别。CLO在10-450和25-500 μ g L ~（-1）范围内线性关系良好（r（2）> 0.9969），EPH在20-450和30-500 μ g L ~（-1）范围内线性关系良好（r（2）> 0.9907）。为了检查该方法的能力，分析了真实的生物样品。结果表明，在定量分析中具有较高的准确度，相对回收率在94.6-105.2%范围内，相对标准偏差小于5.1%，重复性可接受。
In this work, a microfluidic device was developed for on-chip electromembrane extraction of trace amounts of ephedrine (EPH) and clonidine (CLO) in human urine and plasma samples followed by HPLC-UV analysis. Two polymethylmethacrylate plates were used as substrates and a microchannel was carved in each plate. The microchannel channel on the underneath plate provided the flow pass of the sample solution and the one on the upper plate dedicated to a compartment for the stagnant acceptor phase. A piece of polypropylene sheet was impregnated by an organic solvent and mounted between the two parts of the chip device. An electrical field, across the porous sheet, was created by two embedded platinum electrodes placed in the bottom of the channels which were connected to a power supply. The analytes were converted to their ionized form, passed through the supported liquid membrane, and then extracted into the acceptor phase by the applied voltage. All the effective parameters including the type of the SLM, the SLM composition, pH of donor and acceptor phases, and the quantity of the applied voltage were evaluated and optimized. Several organic solvents were evaluated as the SLM to assess the effect of SLM composition. Other parameters were optimized by a central composite design. Under the optimal conditions of voltage of 74 V, flow rate of 28 mu L min(-1), 100 and 20 mM HCl as acceptor and donor phase composition, respectively, the calibration curves were plotted for both analytes. The limits of detection were less than 7.0 and 11 mu g L-1 in urine and plasma, respectively. The linear dynamic ranges were within the range of 10-450 and 25-500 mu g L-1 (r(2) (>) 0.9969) for CLO, and within the range of 20-450 and 30-500 mu g L-1 (r(2) (>) 0.9907) for EPH in urine and plasma, respectively. To examine the capability of the method, real biological samples were analyzed. The results represented a high accuracy in the quantitative" analysis of the analytes with relative recoveries within the range of 94.6-105.2%" and acceptable repeatability with relative standard deviations lower than 5.1%.