Benzene exposure induces gut microbiota dysbiosis and metabolic disorder in mice
Benzene exposure induces gut microbiota dysbiosis and metabolic disorder in mice
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苯暴露导致小鼠肠道菌群失调和代谢紊乱
DOI：
10.1016/j.scitotenv.2019.135879
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发表时间：
2020
影响因子：
9.8
通讯作者：
Pu Yuepu
中科院分区：
文献类型：
--
作者：
Sun Rongli;Xu Kai;Ji Shuangbin;Pu Yunqiu;Man Zhaodi;Ji Jiahui;Chen Minjian;Yin Lihong;Zhang Juan;Pu Yuepu
肠道微生物群包括多物种微生物群落，对维持健康至关重要。苯是一种广泛存在的环境和职业污染物，主要导致血液和骨髓异常。然而，苯对肠道微生物群和代谢的影响尚未研究。本研究以C57 BL/6小鼠为动物模型，分别皮下注射0、6、30和150 mg/kg苯，连续染毒30 d。我们观察到三个苯暴露组的白色细胞水平显着下降，而红细胞和血红蛋白水平仅在30和150 mg/kg苯处理的小鼠中显着变化。16 S rRNA测序结果表明，苯暴露改变了肠道微生物群落的整体结构。此外，在门水平上的放线菌（p<0.05）和属水平上的螺旋菌（p<0.05）在暴露于150 mg/kg苯的小鼠的盲肠内容物和粪便中观察到显著富集。此外，放线菌丰度与血液基本指标（包括白色细胞、红细胞和血红蛋白水平）呈显著负相关。此外，根据LC-MS分析，总共42个盲肠代谢物被150 mg/kg苯显著改变。苯暴露显著影响了几种代谢途径，包括半胱氨酸和蛋氨酸代谢、卟啉和叶绿素代谢、类固醇生物合成、氨酰-tRNA生物合成以及精氨酸和脯氨酸代谢。总之，这项研究表明，苯暴露会导致小鼠肠道微生物群失调和代谢紊乱。
The gut microbiota comprises a multispecies microbial community and is essential for maintaining health. Benzene is a widespread environmental and occupational pollutant that mainly causes blood and bone marrow abnormalities. However, the effects of benzene on gut microbiota and metabolism have not yet been investigated. In this study, C57BL/6 mice were exposed to 0, 6, 30 and 150 mg/kg benzene by subcutaneous injection for 30 days. We observed that white blood cell levels significantly decreased in the three benzene exposure groups, while red blood cell and hemoglobin levels were only changed remarkably in 30 and 150 mg/kg benzene-treated mice. The results of 16S rRNA sequencing showed that benzene exposure altered the overall structure of the gut microbial communities. In addition, significant enrichments ofActinobacteria(p< .05) at the phylum level andHelicobacterat the genus level were observed in the cecal contents and feces of mice exposed to 150 mg/kg benzene. Moreover, there was a significant negative correlation betweenActinobacteriaabundance and basic blood indicators, including white blood cell, red blood cell, and hemoglobin levels. Furthermore, according to LC-MS analysis, a total of 42 cecal metabolites were significantly altered by 150 mg/kg benzene. Several metabolic pathways were significantly influenced by benzene exposure, including cysteine and methionine metabolism, porphyrin and chlorophyll metabolism, steroid biosynthesis, aminoacyl-tRNA biosynthesis, and arginine and proline metabolism. In summary, this study demonstrated that benzene exposure causes dysbiosis of the gut microbiota and metabolic disorder in mice.