A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules
A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules
复制标题
基于四苯乙烯分子聚集和解聚集的碱性磷酸酶和抑制剂筛选的新型荧光启动测定
DOI：
10.1039/c3an36784c
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Deqing
中科院分区：
文献类型：
--
作者：
Gu, Xinggui;Zhang, Guanxin;Zhang, Deqing
考虑到带-PO3H2基团的化合物1可水解成化合物3，而化合物3在水溶液中溶解度低，容易发生聚集，因此用化合物1构建了一种新的碱性磷酸酶（ALP）检测方法。化合物1可以检测低至18 mU mL(-1)浓度的ALP。此外，化合物1已成功应用于活细胞中的ALP检测。此外，化合物 1 可用于筛选 ALP 抑制剂。
Based on the consideration that compound 1 with a -PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL(-1) can be assayed with compound 1. Moreover, compound 1 has been successfully applied for ALP assay in living cells. Also, compound 1 is useful for screening inhibitors of ALP.