BIOCHEMICAL STUDIES OF THE EXCITABLE MEMBRANE OF PARAMECIUM-TETRAURELIA .2. PHOSPHOLIPIDS OF CILIARY AND OTHER MEMBRANES
BIOCHEMICAL STUDIES OF THE EXCITABLE MEMBRANE OF PARAMECIUM-TETRAURELIA .2. PHOSPHOLIPIDS OF CILIARY AND OTHER MEMBRANES
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DOI：
10.1016/0005-2736(79)90205-0
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
NELSON, DL
中科院分区：
文献类型：
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作者：
ANDREWS, D;NELSON, DL
分离和鉴定了松材线虫纤毛和纤毛体的磷脂。主要脂类为1-烷基-2-酰基-3-甘油-3-(2‘-氨基乙基)膦(GAEPL)、磷脂酰乙醇胺和1-烷基-2-酰基-3-甘油-3-磷酰胆碱(GPC)，次要脂类为磷脂酰肌醇、心磷脂、神经酰胺-(2-氨基乙基)磷酸盐(CAEP)、神经酰胺磷乙醇胺(COP)和4种未鉴定的鞘糖脂。纤毛含有4%的心磷脂、15%的GAEPL、41%的磷脂酰乙醇胺、30%的GPC和3%的CAEP和磷脂酰肌醇，纤毛不含心磷脂、24%的GAEPL、37%的磷脂酰乙醇胺、15%的GPC、15%的CAEP、3%的磷脂酰肌醇、2%的COP和少量(约1%)的4种未鉴定的鞘磷脂。在不同生长阶段收获的细胞中，没有发现磷脂成分的变化。同时检测了3种不同表型的6株草履虫突变体的磷脂。突变体的全细胞、纤毛部分和纤毛部分与野生型细胞的类似部分相比只有一个显著的差异：快速突变体FA 97的纤毛部分中有2个额外的少量磷脂(约2%)，初步鉴定为1，2-二酰基-sn-甘油-3-(2‘-氨乙基)磷酸盐(AEPL)和1-alkyl-2-acyl-sn-glycero-3-phosphorylethanolamine(GPE)。
The phospholipids of cilia and deciliated bodies of P. tetraurelia were isolated and characterized. 1-Alkyl-2-acyl-sn-glycero-3-(2''-aminoethyl) phosphonate (GAEPL), phosphatidylethanolamine and 1-alkyl-2-acyl-sn-glycero-3-phosphorylcholine (GPC) were the major lipids and the minor lipids included phosphatidylinositol, cardiolipin, ceramide-(2-aminoethyl) phosphonate (CAEP), ceramide phosphorylethanolamine (COPE) and 4 unidentified sphinogolipids. The deciliated bodies contained 4% cardiolipin, 15% GAEPL, 41% phosphatidylethanolamine, 30% GPC and 3% each of CAEP and phosphatidylinositol; the cilia contained no cardiolipin, 24% GAEPL, 37% phosphatidylethanolamine, 15% GPC, 15% CAEP, 3% phosphatidylinositol, 2% COPE and small amounts (approximately 1%) of the 4 uncharacterized sphingolipids. No alteration in phospholipid composition was found among cells harvested in the various stages of growth. The phospholipids of 6 Paramecium mutants of 3 distinct phenotypes (pawn, paranoiac and fast) were also examined. Only one significant difference was found on comparison of the whole cell, deciliated body and cilia fraction of the mutants with the analogous fractions from wild type cells: the fast mutant, fA 97, had 2 extra, minor phospholipids (approximately 2%) in the deciliated body fraction that were tentatively identified as 1,2-diacyl-sn-glycero-3-(2''-aminoethyl) phosphonate (AEPL) and 1-alkyl-2-acyl-sn-glycero-3-phosphorylethanolamine (GPE).