IMPROVED METHOD FOR EMBEDDING BRAIN-TISSUE IN ALBUMIN-GELATIN
IMPROVED METHOD FOR EMBEDDING BRAIN-TISSUE IN ALBUMIN-GELATIN
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DOI：
10.3109/10520297909112637
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
GOLDMAN, PS
中科院分区：
文献类型：
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作者：
CRANE, AM;GOLDMAN, PS
本文介绍了对Snugras-Dorsey(1963)白蛋白包埋法的一种新的改进。将福尔马林固定的不同年龄恒河猴(Macaca Mulatta)的脑浸泡在含有30%蔗糖的10%磷酸盐缓冲福尔马林中，然后包埋在3%明胶、30%卵白蛋白溶液中，离心以确保均匀。白蛋白明胶在甲醛烟雾中硬化，切成块在10-40微米冷冻。这样制备的部分可以容易地处理和安装，而不会对组织造成损害。对常规细胞和纤维菌株的改良可产生高质量的成品载玻片，其中染色的脑组织被无色白蛋白-明胶基质包围。
A new modification of the Snodgrass-Dorsey (1963) albumin embedding method is described. Formalin fixed brains of various ages of rhesus monkey (Macaca mulatta) were sunk in 10% phosphate buffered formalin which contained 30% sucrose, and then embedded in a 3% gelatin, 30% egg albumin solution which was centrifuged to ensure uniformity. The albumin-gelatin was hardened in formaldehyde fumes and blocks cut frozen at 10-40 .mu.m. Sections thus prepared can be handled easily and mounted without damage to the tissue. Modifications of conventional cell and fiber strains produce high quality finished slides in which the stained brain tissue is surrounded by a colorless albumin-gelatin matrix.