Effect of L-lysine and L-arginine on primary osteoblast cultures from normal and osteopenic rats
Effect of L-lysine and L-arginine on primary osteoblast cultures from normal and osteopenic rats
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DOI：
10.1016/s0753-3322(01)00054-3
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发表时间：
2001-05-01
影响因子：
7.5
通讯作者：
Giardino, R
中科院分区：
文献类型：
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作者：
Fini, M;Torricelli, P;Giardino, R
氨基酸L-赖氨酸(Lys)和L-精氨酸(Arg)对骨质疏松和骨折愈合的治疗作用已被体内研究证实。本研究采用原代培养的成骨细胞，观察氨基酸对细胞基因表达(碱性磷酸酶活性，ALP；骨钙素，OC；I型胶原)、一氧化氮(NO)和细胞增殖的影响。从正常大鼠和骨质疏松症大鼠股骨远端分离细胞。将正常和骨质疏松的骨源性细胞分为4组：对照组、赖氨酸(0.587 mg/m L/d)、精氨酸(0.625 mg/m L/d)和赖氨酸+精氨酸(0.587+0.625 mg/m L/d)。在基础条件下，未观察到正常和骨质疏松的骨源性培养之间的差异。正常成骨细胞经赖氨酸+精氨酸处理7d后，NO生成量较对照组显著增加10.4%(P=0.002)。同时，与对照组相比，Arg组和Lys+Arg组的成骨细胞I型胶原合成分别增加25.3%和28.4%。Lys+Arg作用48h后，骨疏松性成骨细胞的MTT值和细胞计数较对照组分别增加27.6%和28.7%(P=0.002)。与对照组相比，7d时，精氨酸(NO：18.5%；I型胶原：34.4%)和赖氨酸+精氨酸(NO：23.7%；I型胶原：20.9%)组的成骨细胞NO生成和I型胶原合成均显著增加(P<；0.005)。最后，精氨酸处理的骨源性成骨细胞的OC水平显著下降(P=0.025)5.8%。结果提示，Lys和Arg对骨的潜在治疗作用可能至少部分与促进正常骨和骨质疏松骨成骨细胞产生NO和I型胶原合成有关。在骨质疏松的骨源性成骨细胞中，这一合成阶段之前是细胞增殖的初始阶段。(C)2001年版《爱思唯尔科学与医学》。
A therapeutic role of amino acids L-lysine (Lys) and L-arginine (Arg) in osteoporosis and fracture healing was demonstrated previously by in vivo studies. In the present study, primary cultures of osteoblasts were used to investigate the effect of amino acids on gene expression (alkaline phosphatase activity, ALP; osteocalcin, OC; type I collagen), nitric oxide production (NO) and proliferation (MTT) of cells. Cells were isolated from the distal femurs of normal and osteopenic rats. Normal and osteopenic bone-derived cells were divided into four groups: control, Lys (0.587 mg/mL/d), Arg (0.625 mg/mL/d), and Lys + Arg (0.587 + 0.625 mg/mL/d). No evidence of differences between normal and osteopenic bone-derived cultures in basal conditions was observed. A significant (P = 0.002) increase of 10.4% in NO production was observed in normal bone-derived osteoblasts treated with Lys + Arg when compared to the control group at 7 days. At the same time, normal bone-derived osteoblasts treated with Arg and Lys + Arg showed significant increases in type I collagen synthesis of 25.3% and 28.4%, respectively, when compared to the control group. Osteopenic bone-derived osteoblasts showed significant (P = 0.002) increases of 27.6% in MTT and 28.7% in cell count at 48 hours when treated with Lys + Arg in comparison with the control group. At 7 days, NO production and type I collagen synthesis increased significantly (P < 0.005) both in osteopenic bone-derived osteoblasts treated with Arg (NO: 18.5%; type I collagen: 34.4%) and Lys + Arg (NO: 23.7%; type I collagen: 20.9%) compared to the control group. Finally, a significant (P = 0.025) decrease of 5.8% in OC level was observed in osteopenic bone-derived osteoblasts treated with Arg. Results suggest that the potential therapeutic effect of Lys and Arg on bone could be related, at least in part, to an improvement of NO production and type I collagen synthesis by osteoblasts both in normal and in osteopenic bone. In osteopenic bone-derived osteoblasts this synthetic phase is preceded by an initial increase of cell proliferation. (C) 2001 Editions scientifiques et medicales Elsevier SAS.