Leeuwenhoekiella nanhaiensis sp nov., isolated from deep-sea water
Leeuwenhoekiella nanhaiensis sp nov., isolated from deep-sea water
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Leeuwenhoekiella nanhaiensis sp nov.，从深海水中分离
DOI：
10.1099/ijsem.0.000883
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发表时间：
2016-03-01
影响因子：
2.8
通讯作者：
Fang, Jiasong
中科院分区：
文献类型：
--
作者：
Liu, Qianfeng;Li, Jiangtao;Fang, Jiasong
从南海深处采集的水样中分离出一种新型异养、需氧、革兰氏染色阴性、杆状和黄色细菌，命名为菌株 G18(T)。菌株 G18(T) 在 4-40 摄氏度（最佳 28-32 摄氏度）、pH 6.0-8.0（最佳 pH 6.5-7.5）和 0-12 % (w/v) NaCl（最佳 3-4 %）条件下生长。该生物体具有嗜温、耐压的特性，其最适生长压力为0.1 MPa，低于其分离深度。其最适生长温度高于其隔离深度。主要的细胞脂肪酸是C(15:0)iso、C(17:0)iso 3-OH和C(15:1)iso。主要极性脂质由磷脂酰乙醇胺、一种未知氨基脂质和一种未知极性脂质组成。主要呼吸醌是甲基萘醌6。基因组DNA的G+C含量为35mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 G18(T) 与黄杆菌科内已有效公布名称的列文霍基菌 (Leeuwenhoekiella) 属物种聚类，序列相似性为 95.9-98.2%。 DNA-DNA 重关联值范围为 9% 至 42%。差异表型特性以及系统发育的独特性表明菌株 G18(T) 与已有效公布名称的 Leeuwenhoekiella 属物种不同。根据多相证据，菌株G18(T)代表从深海分离的Leeuwenhoekiella属的新物种，其名称为Leeuwenhoekiella nanhaiensis sp。十一月被提议。模式菌株是 G18(T) (=CCTCC AB 2015204(T) =KCTC 42729(T))。
A novel heterotrophic, aerobic, Gram-stain-negative, rod-shaped and yellow bacterium, designated strain G18(T), was isolated from a water sample collected from the deep South China Sea. Strain G18(T) grew at 4-40 degrees C (optimum 28-32 degrees C), at pH 6.0-8.0 (optimum pH 6.5-7.5) and with 0-12 % (w/v) NaCl (optimum 3-4 %). The organism was mesophilic and piezotolerant, its optimal growth pressure was 0.1 MPa, which was lower than that at the depth from which it was isolated. Its optimal growth temperature was higher than that at the depth of its isolation. The predominant cellular fatty acids were C(15 : 0)iso, C(17 : 0)iso 3-OH and C(15 : 1)iso. The major polar lipids were composed of phosphatidylethanolamine, one unknown aminolipid and one unknown polar lipid. The major respiratory quinone was menaquinone 6. The G + C content of the genomic DNA was 35 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain G18(T) clustered with species of the genus Leeuwenhoekiella with validly published names within the family Flavobacteriaceae with 95.9-98.2 % sequence similarity. DNA-DNA reassociation values ranged from 9 to 42 %. Differential phenotypic properties, together with the phylogenetic distinctiveness, suggest that strain G18(T) differs from species of the genus Leeuwenhoekiella with validly published names. On the basis of the polyphasic evidence, strain G18(T) represents a novel species, isolated from deep-sea, of the genus Leeuwenhoekiella for which the name Leeuwenhoekiella nanhaiensis sp. nov. is proposed. The type strain is G18(T) (=CCTCC AB 2015204(T) =KCTC 42729(T)).