Real Time Electronic Feedback for Improved Acoustic Trapping of Micron-Scale Particles
Real Time Electronic Feedback for Improved Acoustic Trapping of Micron-Scale Particles
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DOI：
10.3390/mi10070489
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发表时间：
2019-07-01
期刊：
影响因子：
3.4
通讯作者：
Landers, James P.
中科院分区：
文献类型：
--
作者：
Clark, Charles P.;Farmehini, Vahid;Landers, James P.
声学差异提取以前曾被报道为一种可行的替代重复手动移液和离心步骤，用于从性侵犯样本证据中的女性上皮细胞中分离精子细胞。然而，在含有极大量上皮细胞的样品中，精子细胞分离的效率可能会受到损害。当高浓度样品被裂解时，细胞周围介质的物理化学性质的变化会影响最佳捕获所需的声频。以前的工作已经证明了成功的，自动调整的声频，以考虑在各种样品中的温度和缓冲特性的变化。在这里，我们表明，在声捕获过程中，实时监测电压测量的压电换能器与样品相关的介质中的变化。这是通过宽带峰值检测器电路来实现的，该电路以对施加电压的最小干扰来识别谐振频率。我们进一步证明，立即，相应的调整声捕获频率提供保留精子细胞从高上皮细胞含有模拟性侵犯样本。
Acoustic differential extraction has been previously reported as a viable alternative to the repetitive manual pipetting and centrifugation steps for isolating sperm cells from female epithelial cells in sexual assault sample evidence. However, the efficiency of sperm cell isolation can be compromised in samples containing an extremely large number of epithelial cells. When highly concentrated samples are lysed, changes to the physicochemical nature of the medium surrounding the cells impacts the acoustic frequency needed for optimal trapping. Previous work has demonstrated successful, automated adjustment of acoustic frequency to account for changes in temperature and buffer properties in various samples. Here we show that, during acoustic trapping, real-time monitoring of voltage measurements across the piezoelectric transducer correlates with sample-dependent changes in the medium. This is achieved with a wideband peak detector circuit, which identifies the resonant frequency with minimal disruption to the applied voltage. We further demonstrate that immediate, corresponding adjustments to acoustic trapping frequency provides retention of sperm cells from high epithelial cell-containing mock sexual assault samples.