Feasibility of otolith markings in large juvenile turbot, Scophthalmus maximus, using immersion in alizarin-red S solutions
Feasibility of otolith markings in large juvenile turbot, Scophthalmus maximus, using immersion in alizarin-red S solutions
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DOI：
10.1006/jmsc.2000.0804
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发表时间：
2000-08
影响因子：
4.5
通讯作者：
F. Lagardère;K. Thibaudeau;M. B. Anras
中科院分区：
文献类型：
--
作者：
F. Lagardère;K. Thibaudeau;M. B. Anras
本文分析了茜素红S（ARS）浸泡在大型幼鱼耳石中产生标记的效率。使用ARS海水溶液的四种处理（缓冲ARS 100、200、400 mg l-1和未缓冲ARS 400 mg l-1）标记涡轮机（总长约136 mm，湿重45 g）24 h。使用茜素络合酮（AC）的未缓冲海水溶液（从初步试验设定为120 mg l-1）的单次处理作为参考标记。所有的鱼都存活到实验结束（标记后33天），它们的生长速度在组间没有显著差异。在紫外光显微镜下观察耳石的正面切片，发现ARS和AC分别引起黄色和粉红色的标记。在ARS浓度为400 mg l-1时，AC和ARS浸泡的标记质量相同。ARS浸泡具有双重优势，是一个廉价的和（可能）低应力的技术标记大菱鲆幼鱼的耳石。建议将ARS应用于其他海洋和淡水物种的大型幼鱼。
The efficiency with which immersion in alizarin red S (ARS) produced marks in the otoliths of large juvenile fish was analysed. Turbots (approximately 136 mm total length and 45 g wet weight) were marked for 24 h using four treatments of seawater solutions of ARS (buffered ARS 100, 200, 400 mg l −1 and unbuffered ARS 400 mg l −1 ). A single treatment with an unbuffered seawater solution of alizarin complexone (AC) (set to 120 mg l −1 from preliminary trials) was used as a reference marker. All fish survived to the end of the experiment (33 days after marking), and their growth rates did not differ significantly between groups. Examination of frontal sections of otoliths under UV light microscopy revealed the yellowish and pink marks induced by ARS and AC, respectively. The quality of marks was equal for both AC and ARS immersions, at a 400 mg l −1 concentration of ARS. ARS immersion has the double advantage of being an inexpensive and (probably) low-stress technique for marking the otoliths of large turbot juveniles. The application of ARS to large juveniles of other marine and freshwater species is suggested.