Health Effects of Dietary Oxidized Milk Administration in Offspring Mice during Pregnancy and Lactation with Metabolomic Strategies.
Health Effects of Dietary Oxidized Milk Administration in Offspring Mice during Pregnancy and Lactation with Metabolomic Strategies.
复制标题
DOI：
10.1021/acs.jafc.1c07132
复制
发表时间：
2022-02
影响因子：
6.1
通讯作者：
Ling Mo;Chaochao Zhao;B. Huang;Jiawei Niu;Siyan Hong;Jing-Jing Li-Jing;Yintao Lin;Fengqiong Qin
中科院分区：
文献类型：
--
作者：
Ling Mo;Chaochao Zhao;B. Huang;Jiawei Niu;Siyan Hong;Jing-Jing Li-Jing;Yintao Lin;Fengqiong Qin
牛奶是怀孕期间重要的营养来源。先前的研究一致表明，牛奶和乳制品中的氧化会诱导肝脏和肾脏的氧化应激、炎症和纤维化。然而，这些影响背后的机制在很大程度上仍未被探索。研究氧化乳对仔鼠粪便代谢及肝肾功能的影响。采用H2 O2-Cu或加热对牛奶进行氧化改性，造成不同程度的氧化损伤。昆明雌性小鼠用H2 O2-Cu、热或正常对照饲料喂养，直至其后代3周龄。收集粪便用于基于质谱法的代谢组学研究。筛选了42种潜在的重要代谢生物标志物，并比较了各组的相对强度。结果表明，氧化乳主要调节异亮氨酸代谢、脯氨酸代谢和三羧酸循环。此外，组织病理学分析显示，摄入氧化牛奶后，蛋白质和脂质氧化产物在肝脏和肾脏组织中积累，从而诱导氧化应激，增加炎症因子水平，并显著增加炎症通路相关基因和蛋白质的表达。上述结果表明，妊娠期摄入氧化乳可能会通过干扰氨基酸和能量代谢，增加雄性后代肝脏和肾脏损伤的风险，凸显了氧化乳对人类的潜在健康风险。
Milk is an important source of nutrients during pregnancy. Previous studies have consistently shown that oxidation in milk and dairy products can induce oxidative stress, inflammation, and fibrosis in the liver and kidney. However, the mechanism underlying these effects remains largely unexplored. This study aimed to investigate the effects of oxidized milk on fecal metabolism and liver and kidney function of offspring mice. Oxidative modification of milk was performed using H2O2-Cu or heating, causing varying degrees of oxidative damage. Kunming female mice were fed with a H2O2-Cu, heat, or normal control diet until their offspring were 3 weeks old. Feces were collected for the metabolomics study based on mass spectrometry. Forty-two potentially significant metabolic biomarkers were screened, and each group's relative intensity was compared. The results showed that oxidized milk mainly regulated isoleucine metabolism, proline metabolism, and tricarboxylic acid cycle. In addition, the histopathological analysis showed accumulation of protein and lipid oxidation products in the liver and kidney tissues after intake of oxidized milk, which induced oxidative stress, increased the levels of inflammatory factors, and significantly increased the expression of genes and proteins involved in inflammatory pathways. The above results suggest that intake of oxidized milk during gestation may increase the risk of liver and kidney injury in male offspring by interfering with amino acid and energy metabolism, highlighting the potential health risks of oxidized milk in humans.