Expression of luminal and basal cytokeratins in human breast carcinoma
Expression of luminal and basal cytokeratins in human breast carcinoma
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DOI：
10.1002/path.1559
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发表时间：
2004-06-01
影响因子：
7.3
通讯作者：
Ellis, IO
中科院分区：
文献类型：
--
作者：
El-Rehim, DMA;Pinder, SE;Ellis, IO
我们研究了1944例浸润性乳腺癌中基底细胞和腔细胞角蛋白的表达，使用组织微阵列（TMA）技术，以确定每个细胞角蛋白亚型的表达频率，它们之间的关系和预后相关性，如果有的话。使用管腔细胞角蛋白（CK）7/8、18和19以及基础标志物CK 5/6和CK 14的抗体，通过免疫细胞化学染色测定表达。此外，还评估了α-平滑肌肌动蛋白（SMA）和雌激素受体状态（ER）。绝大多数病例显示CK 7/8、18和19阳性，表明分化的腺体表型，这一发现与良好的预后、ER阳性和患者年龄较大相关。相反，基础标志物表达与不良预后、ER阴性和年轻患者年龄显著相关。多因素分析显示CK 5/6是无复发间期的独立指标。我们能够亚组的情况下，分为四个不同的表型类别（纯管腔，混合管腔/基底，纯基底和空），这有显着差异的生物学特征和临床病程的疾病。分类为表达基底表型的肿瘤（管腔+基底和纯基底）属于预后不良亚组，大多数情况下通常为ER阴性。这些发现提供了进一步的证据，表明乳腺癌具有不同的分化亚类，具有生物学和临床相关性。版权所有（C）2004大不列颠和爱尔兰病理学会。出版社：John Wiley Sons，Ltd
We have examined basal and luminal cell cytokeratin expression in 1944 cases of invasive breast carcinoma, using tissue microarray (TMA) technology, to determine the frequency of expression of each cytokeratin subtype, their relationships and prognostic relevance, if any. Expression was determined by immunocytochemistry staining using antibodies to the luminal cytokeratins (CKs) 7/8, 18 and 19 and the basal markers CK 5/6 and CK 14. Additionally, assessment of alpha-smooth muscle actin (SMA) and oestrogen receptor status (ER) was performed. The vast majority of the cases showed positivity for CK 7/8, 18 and 19 indicating a differentiated glandular phenotype, a finding associated with good prognosis, ER positivity and older patient age. In contrast, basal marker expression was significantly related to poor prognosis, ER negativity and younger patient age. Multivariate analysis showed that CK 5/6 was an independent indicator for relapse free interval. We were able to subgroup the cases into four distinct phenotype categories (pure luminal, mixed lumina/basal, pure basal and null), which had significant differences in relation to the biological features and the clinical course of the disease. Tumours classified as expressing a basal phenotype (the combined luminal plus basal and the pure basal) were in a poor prognostic subgroup, typically ER negative in most cases. These findings provide further evidence that breast cancer has distinct differentiation subclasses that have both biological and clinical relevance. Copyright (C) 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley Sons, Ltd.