Single-Cell Behavioral Assays for Heterogeneity Studies
Single-Cell Behavioral Assays for Heterogeneity Studies
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DOI：
10.1007/978-3-662-49118-8_1
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Yoon, Euisik
中科院分区：
文献类型：
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作者：
Chen, Yu-Chih;Ingram, Patrick;Yoon, Euisik
细胞异质性是细胞生物学和癌症治疗中的新挑战。异质群体中的每个细胞都有自己独特的行为，因此对相同的试剂或药物有不同的反应，使得分析或治疗变得困难和复杂。因此，了解细胞的异质性特征在表型行为测定中是重要的。尽管传统的荧光激活细胞分选可以基于标记物分离细胞，但大多数测定仅呈现通过标记物分选的一批细胞中的平均行为。重要的是要有能力在小型化，高通量平台中充分表征单个细胞的行为，区分它们的异质性并随时间跟踪它们的反应。本章介绍了实现这些测定和相关生物学实验的技术创新，包括（1）单细胞分离和跟踪方案，以研究细胞增殖，细胞分化和谱系，（2）细胞迁移平台，以测量细胞运动，变形和侵袭力，（3）细胞与细胞相互作用平台，研究细胞之间相互作用引起的细胞行为改变。
Cell heterogeneity is an emerging challenge in cell biology and cancer therapies. Each cell in heterogeneous populations has its own unique behavior, and thus responds differently to the same reagent or drug, making analysis or treatment difficult and complicated. Therefore, it is important to understand the heterogeneity characteristics of cells in phenotypic behavior assays. Although conventional fluorescence-activated cell sorting can separate cells based on markers, most assays simply present the average behavior in a batch of cells sorted by markers. It is important to have a capability to fully characterize individual cell behaviors in a miniaturized, high-throughput platform, distinguishing their heterogeneity and tracking their responses over time. This chapter introduces the technological innovations enabling these assays and the associated biological experiments, including (1) single-cell isolation and tracking schemes to investigate the cell proliferation, cell differentiation and lineage, (2) cell migration platforms to measure cell motility, deformation, and invasiveness, and (3) cell-cell interaction platforms to study the alteration of cell behaviors caused by reciprocal interactions among cells.