Co-expressed recombinant human Translin-Trax complex binds DNA
Co-expressed recombinant human Translin-Trax complex binds DNA
复制标题
DOI：
10.1016/j.febslet.2005.05.007
复制
发表时间：
2005-06-06
期刊：
影响因子：
3.5
通讯作者：
Rao, BJ
中科院分区：
文献类型：
--
作者：
Gupta, GD;Makde, RD;Rao, BJ
单独表达的Trax聚集成不溶性复合物，而在与翻译蛋白共表达时变得容易溶解并形成稳定的异聚复合物（类似于430 kDa），其含有几乎等摩尔比的两种蛋白质。基于通过MALDI-TOF-MS估计的亚基分子量，纯化的复合物似乎包含八聚体翻译蛋白加六聚体Trax（计算的MW 420 kDa）或Trax和翻译蛋白各自的七聚体（计算的MW 425 kDa）或六聚体翻译蛋白加八聚体Trax（计算的MW 431 kDa）。该复合物结合单链/双链DNA。ssDNA凝胶移位复合物显示两种蛋白质几乎等摩尔比，表明翻译蛋白“伴侣”Trax和形成具有DNA结合能力的异聚复合物。(c)2005年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Trax, expressed alone aggregates into insoluble complexes, whereas upon co-expression with Translin becomes readily soluble and forms a stable heteromeric complex (similar to 430 kDa) containing both proteins at nearly equimolar ratio. Based on the subunit molecular weights, estimated by MALDI-TOF-MS, the purified complex appears to comprise of either an octameric Translin plus a hexameric Trax (calculated MW 420 kDa) or a heptamer each of Trax and Translin (calculated MW 425 kDa) or a hexameric Translin plus an octameric Trax (calculated MW 431 kDa). The complex binds single-stranded/double-stranded DNA. ssDNA gel-shifted complex shows both proteins at nearly equimolar ratio, suggesting that Translin "chaperones" Trax and forms heteromeric complex that is DNA binding competent. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.