Effect of Y-27632 on the cultured retinal neurocytes of rats
Effect of Y-27632 on the cultured retinal neurocytes of rats
复制标题
Y-27632对培养的大鼠视网膜神经细胞的影响。
DOI：
10.3980/j.issn.2222-3959.2013.01.03
复制
发表时间：
2013-02-18
影响因子：
1.4
通讯作者：
Zhong, Yi-Sheng
中科院分区：
文献类型：
--
作者：
Feng, Pei-Li;Wang, Jing;Zhong, Yi-Sheng
目的：研究Y-27632对体外培养的视网膜神经细胞存活和突起生长的影响。出生后第2 - 3天，将Sprague -道利视网膜神经细胞培养48小时，更换为无血清培养基（对照组）和含有30 μ mol/L Y-27632的无血清培养基（Y-27632组），并将细胞继续培养另外48小时。用抗神经元特异性烯醇化酶（NSE）免疫细胞化学方法对培养的视网膜神经细胞进行鉴定。结果：与对照组相比，Y-27632组细胞在培养72和96 h后，细胞存活吸光度值分别增加12.90%和33.33%; Y-27632对培养的视网膜神经细胞直径无明显影响。与对照组相比，Y-27632在培养72和96 h后可稳定促进视网膜神经细胞突起生长（P=0.001）。结论：Y-27632可促进出生后早期培养视网膜神经细胞的存活和突起生长。
AIM: To investigate the effect of Y -27632 on the survival and neurite outgrowth of the cultured retinal neurocytes.METHODS: After the postnatal day 2 -3, Sprague - Dawley retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum -free media (control group) and serum-free media contained 30 mu mol/L Y-27632 (Y-27632 group), and the cells were continually cultured another 48 hours. The cultured retinal neurocytes were identified with anti -neuron specific enolase (NSE) immunocytochemistry. The survival state of those cells was estimated by MTT assay, and the neurite outgrowth of those cells was evaluated by the computerized image-analysis system.RESULTS: Compared with the control group, the absorbance values of cells survival in Y -27632 group increased 12.90% and 33.33% respectively after 72 and 96 hours culture. Y -27632 had no significant effect on the diameter of cultured retinal neurocytes. Compared with the control group, Y -27632 induced a stable improvement of neurite outgrowth of retinal neurocytes after 72 and 96 hours culture (P=0.001).CONCLUSION: Y -27632 could promote the survival and neurite outgrowth of the early postnatal cultured retinal neurocytes.