In vivo photoacoustic imaging of chorioretinal oxygen gradients
In vivo photoacoustic imaging of chorioretinal oxygen gradients
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DOI：
10.1117/1.jbo.23.3.036005
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发表时间：
2018-03-01
影响因子：
3.5
通讯作者：
Jokerst, Jesse V.
中科院分区：
文献类型：
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作者：
Hariri, Ali;Wang, Junxin;Jokerst, Jesse V.
脉络膜视网膜成像对脉络膜视网膜血管性疾病如新生血管性年龄相关性黄斑变性的诊断具有重要作用。眼部氧梯度成像可以更好地诊断和治疗眼部疾病。在此，我们描述了光声眼部成像（PAOI）在测量新西兰白色兔（n = 5）眼部缺血时脉络膜视网膜氧饱和度（CR - sO（2））梯度中的应用。我们观察到脉搏血氧饱和度和PAOI之间具有良好的相关性（R-2 = 0.98），作为吸入空气中不同氧气百分比的函数。然后，我们使用建立的眼缺血模型，其中眼内压升高以收缩眼血流，并且注意到磷酸盐缓冲盐水（PBS）的注射体积与眼内压（IOP）之间的正相关（R-2 = 0.92）以及CR-sO2与PBS的注射体积之间的负相关（R-2 = 0.98）。在注射前（基线）、注射中（缺血）和注射后（600 μ L PBS）测量CR-sO（2）.缺血-再灌注模型不影响在动物爪上使用脉搏血氧仪测量sO（2），但脉络膜视网膜PAOI信号显示CR-sO（2）降低近6倍（n = 5，p = 0.00001）。我们还观察到，在缺血期间IOP显著升高后，CR-sO（2）降低6倍，在再灌注期间接近基线增加。这些数据表明，PAOI可以检测脉络膜视网膜氧合的变化，并可能是有用的应用程序，以成像氧气梯度在眼部疾病。(C)2018年，摄影光学仪器工程师协会（SPIE）
Chorioretinal imaging has a crucial role for the patients with chorioretinal vascular diseases, such as neovascular age-related macular degeneration. Imaging oxygen gradients in the eye could better diagnose and treat ocular diseases. Here, we describe the use of photoacoustic ocular imaging (PAOI) in measuring chorioretinal oxygen saturation (CR - sO(2)) gradients in New Zealand white rabbits (n = 5) with ocular ischemia. We observed good correlation (R-2 = 0.98) between pulse oximetry and PAOI as a function of different oxygen percentages in inhaled air. We then used an established ocular ischemia model in which intraocular pressure is elevated to constrict ocular blood flow, and notice a positive correlation (R-2 = 0.92) between the injected volume of phosphate buffered saline (PBS) and intraocular pressure (IOP) as well as a negative correlation (R-2 = 0.98) between CR - sO2 and injected volume of PBS. The CR - sO(2) was measured before (baseline), during (ischemia), and after the infusion (600-mu L PBS). The ischemia-reperfusion model did not affect the measurement of the sO(2) using a pulse oximeter on the animal's paw, but the chorioretinal PAOI signal showed a nearly sixfold decrease in CR - sO(2) (n = 5, p = 0.00001). We also observe a sixfold decrease in CR - sO(2) after significant elevation of IOP during ischemia, with an increase close to baseline during reperfusion. These data suggest that PAOI can detect changes in chorioretinal oxygenation and may be useful for application to imaging oxygen gradients in ocular disease. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)