AGE-RELATED DIFFERENCES IN RECEPTORS FOR ESCHERICHIA-COLI HEAT-STABLE ENTERO-TOXIN IN THE SMALL AND LARGE-INTESTINE OF CHILDREN
AGE-RELATED DIFFERENCES IN RECEPTORS FOR ESCHERICHIA-COLI HEAT-STABLE ENTERO-TOXIN IN THE SMALL AND LARGE-INTESTINE OF CHILDREN
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DOI：
10.1016/0016-5085(88)90423-4
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发表时间：
1988-02-01
期刊：
影响因子：
29.4
通讯作者：
GIANNELLA, RA
中科院分区：
文献类型：
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作者：
COHEN, MB;GUARINO, A;GIANNELLA, RA
大肠杆菌产生热稳定肠毒素是一种世界性的肠道疾病，特别是在儿童中。我们检查了不同年龄儿童的小肠和大肠标本中是否存在E。大肠杆菌热稳定肠毒素受体，并确定是否受体的数量或这些受体的结合亲和力与儿童的年龄有关。我们观察到特异性结合125 I-热稳定肠毒素的所有小肠和结肠标本。然而，在婴儿中，每微克膜蛋白的受体数量更多，并且随着年龄的增长，受体数量迅速减少。我们还观察到鸟苷酸环化酶的热稳定肠毒素刺激增加与受体密度增加相关。我们认为，更多的热稳定肠毒素的胃肠道受体，能够激活更多的鸟苷酸环化酶，可能有助于增加腹泻的严重程度在幼儿暴露于肠毒素E。杆菌
Escherichia coli that produce heat-stable enterotoxin are a worldwide cause of diarrheal disease, especially in children. We examined small and large intestinal specimens from children of various ages for the presence of E. coli heat-stable enterotoxin receptors and determined whether the number of receptors or the binding affinity of these receptors was related to the age of the child. We observed specific binding of 125I-heat-stable enterotoxin to all small intestinal and colonic specimens. However, a greater number of receptors per microgram of membrane protein were present in infants and the number of receptors rapidly decreased with increasing age. We also observed that increased heat-stable enterotoxin stimulation of guanylate cyclase was correlated with increased receptor density. We suggest that a greater number of gastrointestinal receptors for heat-stable enterotoxin, capable of activating more guanylate cyclase, may contribute to the increased severity of diarrhea noted in young children exposed to enterotoxigenic E. coli.