CHROMOSOMAL LOCATION OF GENES PARTICIPATING IN DEGRADATION OF PURINES IN PSEUDOMONAS-AERUGINOSA
CHROMOSOMAL LOCATION OF GENES PARTICIPATING IN DEGRADATION OF PURINES IN PSEUDOMONAS-AERUGINOSA
复制标题
DOI：
10.1007/bf00266910
复制
发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
TERAWAKI, Y
中科院分区：
文献类型：
--
作者：
MATSUMOTO, H;OHTA, S;TERAWAKI, Y
对铜绿假单胞菌菌株PAO中编码催化嘌呤降解的酶的基因（Puu）进行遗传作图。分离腺嘌呤脱氨酶（puuA）、鸟嘌呤脱氨酶（puuB）、黄嘌呤脱氢酶（puuC）、尿酸酶（puuD）、尿囊素酶（puuE）和/或尿囊素酶（puuF）缺陷的突变体并用于遗传研究。通过FP 5因子的缀合和通过噬菌体G101的一般化转导，得到这6个基因在染色体上的以下图谱位置：hisI-puuB-hisII; trpA，B-puuA-ilv202; met9011-catA1-tyu-nar9011-（puuC，puuD，puuE）-puF。puuC，puuD和PuuE之间的紧密连锁被证明的转导。
Genetic mapping of the genes (Puu) that encode the enzymes catalyzing degradation of purines in P. aeruginosa strain PAO was carried out. Mutants that are deficient in adenine deaminase (puuA), guanine deaminase (puuB), xanthine dehydrogenase (puuC), uricase (puuD), allantoinase (puuE) and/or allantoicase (puuF) were isolated and used for the genetic study. Conjugation by FP5 factor and generalized transduction by phage G101 gave the following map locations of these 6 genes on the chromosome: hisI-puuB-hisII; trpA,B-puuA-ilv202; met9011-catA1-tyu-nar9011 - (puuC, puuD, puuE)-puuF. A close linkage among the puuC, puuD and PuuE was demonstrated by the transduction.