Reclustering of scattered Golgi elements occurs along microtubules.
Reclustering of scattered Golgi elements occurs along microtubules.
复制标题
分散的高尔基体元素沿着微管重新聚集。
DOI：
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发表时间：
1989
影响因子：
6.6
通讯作者：
T. Kreis
中科院分区：
文献类型：
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作者：
Wai Chang Ho;Victoria J. Allan;G. V. Meer;E. Berger;T. Kreis
诺考达唑引起的间期微管解聚导致Vero成纤维细胞高尔基体的分散和明显的碎片化。去除药物后，间期微管reflection，分散的高尔基体元素移动回到周围的微管组织中心（MTOC）的区域在40至60分钟内。使用荧光脂质类似物（C6-NBD-神经酰胺）作为分散的高尔基体元素的重要染色，他们的搬迁可视化视频增强荧光显微镜在Vero细胞保持在20摄氏度。NBD标记的结构被确定为高尔基体元件的共定位与半乳糖基转移酶在固定的细胞。在重新聚类过程中，NBD标记的高尔基体元件被观察到以0.1至0.4微米/秒的速度向MTOC不连续跳跃移动。高尔基体分子移动的沿着路径重叠在微管上，通过间接免疫荧光观察。无论是崩溃的中间丝所造成的显微注射的抗体波形蛋白，也没有破坏微丝细胞松弛素D的重新聚集的高尔基体元件或定位的高尔基体的影响。这些数据表明，分散的高尔基体元件移动沿着微管周围的MTOC的区域，而既不完整的中间丝，也不涉及微丝。
Depolymerization of the interphase microtubules by nocodazole results in the scattering and apparent fragmentation of the Golgi apparatus in Vero fibroblast cells. Upon removal of the drug, the interphase microtubules repolymerize, and the scattered Golgi elements move back to the region around the microtubule-organizing center (MTOC) within 40 to 60 min. Using a fluorescent lipid analogue (C6-NBD-ceramide) as a vital stain for the scattered Golgi elements, their relocation was visualized by video-enhanced fluorescence microscopy in Vero cells maintained at 20 degrees C. The NBD-labeled structures were identified as Golgi elements by their colocalization with galactosyltransferase in the fixed cells. During reclustering, NBD-labeled Golgi elements were observed to move by discontinuous saltations towards the MTOC with velocities of 0.1 to 0.4 micron/s. Paths along which Golgi elements moved were super-imposable on microtubules visualized by indirect immunofluorescence. Neither the collapse of intermediate filaments caused by microinjection of antibodies to vimentin nor the disruption of microfilaments by cytochalasin D had an effect on the reclustering of Golgi elements or the positioning of the Golgi apparatus. These data show that scattered Golgi elements move along microtubules back to the region around the MTOC, while neither intact intermediate filaments nor microfilaments are involved.