A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis
A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis
复制标题
一种共表达策略，可实现二聚体蛋白内单个亚基的标记以进行单分子分析
DOI：
10.1039/c7cc03032k
复制
发表时间：
2017
影响因子：
4.9
通讯作者：
Perrett Sarah
中科院分区：
文献类型：
--
作者：
Lou Fei;Yang Jie;Wu Si;Perrett Sarah
一种通用的共表达策略，用于将单个供体-受体染料对位点特异性掺入二聚体蛋白质的任何位置，允许对以前无法使用该技术的蛋白质进行单分子FRET研究，例如在其球状二聚体C结构域的背景下的Ure 2的固有无序朊病毒N结构域。
A generic co-expression strategy for site-specific incorporation of a single donor–acceptor dye pair into any position in a dimeric protein, allowing single molecule FRET study of proteins previously inaccessible to this technique, such as the intrinsically disordered prion N-domain of Ure2 in the context of its globular dimeric C-domain.