INCREASED LEVELS OF ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT MESSENGER-RNA IN EXPERIMENTAL AUTOIMMUNE MYASTHENIA-GRAVIS
INCREASED LEVELS OF ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT MESSENGER-RNA IN EXPERIMENTAL AUTOIMMUNE MYASTHENIA-GRAVIS
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DOI：
10.1016/0014-5793(88)80442-3
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发表时间：
1988-06-20
期刊：
影响因子：
3.5
通讯作者：
FUCHS, S
中科院分区：
文献类型：
--
作者：
ASHER, O;NEUMANN, D;FUCHS, S
为了深入了解乙酰胆碱受体在肌无力中阻断和丢失的调控机制，我们观察了肌无力和正常兔和大鼠腿部肌肉中乙酰胆碱受体α亚单位基因的表达水平。将来自正常和肌无力动物的RNA的Northern blotting与小鼠AChRα亚单位的cDNADNA探针杂交。我们的实验表明，与对照组动物相比，实验性自身免疫性重症肌无力动物α亚单位基因表达水平显著增加(4-7倍)。肌动蛋白信使核糖核酸水平基本不变。因此，我们的结果提示，EAMG伴随着AChR基因转录水平的升高。
To gain insight into the regulatory mechanisms underlying the blockade and loss of acetylcholine receptor (AChR) in myasthenia, we have followed AChR α‐subunit mRNA levels in leg muscles of myasthenic and normal rabbits and rats. Northern blots of RNA preparations from normal and myasthenic animals were hybridized with a mouse AChR α‐subunit cDNA probe. Our experiments indicate a specific increase (4–7‐fold) in the levels of α‐subunit mRNA in animals with experimental autoimmune myasthenia gravis (EAMG), in comparison with control animals. Actin mRNA levels were essentially unchanged. Our results thus suggest that EAMG is accompanied by an increased level of AChR gene transcription.