Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe
Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe
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DOI：
10.2741/2835
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发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Provost, Patrick
中科院分区：
文献类型：
--
作者：
Gobeil, Lise-Andree;Plante, Pierre;Provost, Patrick
核糖核酸酶III Dicer（Dcr 1）已被证明是裂殖酵母染色体分离和基因沉默所必需的。这些作用被认为是转录的，由异染色质的形成和维持介导，并由来自Dcr 1的小RNA引导，沿着称为RNA干扰的过程。为了进一步了解Dcr 1的基因调控作用，我们对dcr 1敲除和野生型裂殖酵母菌株进行了比较分析。部分可溶性蛋白质组的分析确定了8个细胞蛋白的表达下Dcr1控制，其中三个是糖酵解途径的组成部分。与它们各自的mRNA转录水平的进一步相关性与涉及Dcr 1或Dcr 1复合物的转录后基因调控机制的存在是相容的。实验设计，以确定Dcr 1复合物的成分揭示了两个新的Dcr 1相互作用，即锌指蛋白Byr 3和核糖体蛋白L12。Byr 3和L12可能分别与Dcr 1的转录和翻译机制相连接，并参与了裂殖酵母的转录后基因调控。
The ribonuclease III Dicer ( Dcr1) has been shown to be required for chromosome segregation and gene silencing in Schizosaccharomyces pombe. These effects are thought to be transcriptional, mediated by formation and maintenance of heterochromatin, and guided by small RNAs derived from Dcr1 along a process known as RNA interference. In order to get further insights into the gene regulatory role of Dcr1, we performed comparative analyses of dcr1 knockout and wild-type fission yeast strains. Analysis of part of the soluble proteomes identified eight cellular proteins whose expression is under Dcr1 control, three of which are integral constituents of the glycolysis pathway. Further correlations with their respective mRNA transcript levels are compatible with the existence of a post-transcriptional gene regulatory mechanism involving Dcr1 or a Dcr1 complex. Experiments designed to identify components of Dcr1 complexes unveiled two novel Dcr1 interactors, namely the zinc finger protein Byr3 and the ribosomal protein L12. Consistently enriched in Dcr1 immune complexes, Byr3 and L12 may link Dcr1 to the transcriptional and translational machineries, respectively, and contribute to post-transcriptional gene regulation in fission yeast.