Molecular identification of species and ploidy of Carassius fishes in Lake Biwa, using mtDNA and microsatellite multiplex PCRs
Molecular identification of species and ploidy of Carassius fishes in Lake Biwa, using mtDNA and microsatellite multiplex PCRs
复制标题
DOI：
10.1007/s10228-014-0388-9
复制
发表时间：
2014
影响因子：
1.2
通讯作者：
Tappei Mishina;Mikumi Takada;H. Takeshima;M. Nakano;Ryoichi Tabata;M. Nishida;Katsutoshi Watanabe
中科院分区：
文献类型：
--
作者：
Tappei Mishina;Mikumi Takada;H. Takeshima;M. Nakano;Ryoichi Tabata;M. Nishida;Katsutoshi Watanabe
建立了一种高效可靠的琵琶湖鲫鱼种类和倍性鉴定方法。利用多重线粒体DNA单倍型特异性PCR和基于核DNA微卫星PCR的倍性测定相结合的方法对布氏菌（buergeri，后者的多倍体）进行鉴定。流式细胞术测定倍性的准确性较高。该方法还可以鉴定三倍体无性系，并提供其遗传多样性和结构信息。该方法适用于琵琶湖鲫鱼整个生活史的生态学和进化研究。
We established an efficient and reliable method to identify the species and ploidy ofCarassiusfishes in Lake Biwa (C.cuvieri,C.buergeri, and the latter’s polyploid) using a combination of multiplex mitochondrial DNA haplotype-specific PCR and ploidy determination based on nuclear DNA microsatellite PCR. The high accuracy of ploidy determination was confirmed by flow cytometry. This method can also identify triploid clonal lines and provide information about their genetic diversity and structure. The method is applicable to ecological and evolutionary studies ofCarassiusfishes in Lake Biwa throughout their entire life history.